Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-HLA E 抗体 [MEM-E/07] (ab11820)

Reviews (3)Q&A (1)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)
  • Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)

Key features and details

  • Mouse monoclonal [MEM-E/07] to HLA E
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HLA E antibody [MEM-E/07]
    HLA E 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-E/07] to HLA E

  • 由来種

    Mouse

  • 特異性

    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).

  • アプリケーション

    適用あり: Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to HLA E. The epitope is extracellular. The antibody reacts only with native epitope, not denatured.
    Database link: P13747

  • ポジティブ･コントロール

    • IHC-P: Human spleen tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified from hybridoma culture supernatant by protein A-affinity chromatography.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-E/07

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11820の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (1)
Use a concentration of 1 - 4 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
特記事項
Flow Cyt
Use a concentration of 1 - 4 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性

    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

  • 細胞内局在

    Membrane; Single-pass type I membrane protein

  • 参照データベース

    • 別名

      • HLA class I histocompatibility antigen E alpha chain antibody
      • EA1.2 antibody
      • EA2.1 antibody
      • HLA 6.2 antibody
      • HLA class I histocompatibility antigen alpha chain E antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA6.2 antibody
      • HLAE antibody
      • Lymphocyte antigen antibody
      • Major histocompatibility complex class I E antibody
      • MHC antibody
      • MHC class I antigen E antibody
      • MHC HLA E alpha 1 antibody
      • MHC HLA E alpha 2.1 antibody
      • QA1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)

      Immunohistochemistry parafin embedded sections staining of huam spleen tissue using ab11820.

    • Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)
      Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)

      Flow cytometry analysis showing separation of HLA-E transfected LCL cells stained using ab11820 (concentration in sample 2 μg/ml, red-filled) from HLA-E transfected LCL cells stained using mouse IgG1 isotype control purified antibody (concentration in sample 2 μg/ml, same as HLA-E purified antibody concentration, black-dashed).

    参考文献 (8)

    ab11820 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab11820 は 8 報の論文で使用されています。

    • Samiec M  et al. The Relative Abundances of Human Leukocyte Antigen-E, α-Galactosidase A and α-Gal Antigenic Determinants Are Biased by Trichostatin A-Dependent Epigenetic Transformation of Triple-Transgenic Pig-Derived Dermal Fibroblast Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36142211
    • Sasaki T  et al. Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells. Eur J Immunol 46:929-40 (2016). PubMed: 26711740
    • Shwetank  et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). PubMed: 25086398
    • Shwetank  et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . PubMed: 24236107
    • Jagessar SA  et al. Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG34-56 peptide in incomplete adjuvant. Eur J Immunol 42:217-27 (2012). PubMed: 21928277
    • Jørgensen PB  et al. Epstein-Barr virus Peptide Presented by HLA-E is Predominantly Recognized by CD8(bright) Cells in multiple Sclerosis Patients. PLoS One 7:e46120 (2012). Flow Cyt . PubMed: 23049954
    • Park B  et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . PubMed: 20713594
    • Palmisano GL  et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA E antibody [MEM-E/07]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Sample
    Human Cell (Human endothelial cell line)
    Specification
    Human endothelial cell line
    Permeabilization
    Yes - 0.1% triton-x 100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 24 2013

    Western blot abreview for Anti-HLA E antibody [MEM-E/07]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelial)
    Loading amount
    100 µg
    Specification
    endothelial
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 27 2012

    Flow Cytometry abreview for Anti-HLA E antibody [MEM-E/07]

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Human Endothelial cell line (ECV304))
    Specification
    Human Endothelial cell line (ECV304)
    Preparation
    Cell harvesting/tissue preparation method: Trypsinisation
    Sample buffer: PVG containing 0.1% trypsin
    Fixation
    2% Paraformaldehyde in PBS
    Permeabilization
    No
    Gating Strategy
    total population
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Shwetank Verma

    Verified customer

    投稿 Mar 16 2011

    Question

    I have used the HLA-E Ab MEM-E/07 (ab11820) for a work. The referees of my manuscript ask me to add a reference (published results) to clarify the specificity of the HLA-E Ab MEM-E/07 (ab11820). Can you help me.

    Read More
    Answer

    I am pleased to be able to provide you with two references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456 If you would like the pdf of the Palmisano paper I can send it to you. I hope this will help you and wish you all the best with your paper. Could you please let us know when you have published it so we can add your reference to the antibody's datasheet, and/or consider submitting an Abreview with your experience of the antibody (we reward Abreviews with points which you can use for future discounts and gifts)?

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com