Anti-HLA E 抗体 (ab88090)
Key features and details
- Mouse polyclonal to HLA E
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HLA E antibody
HLA E 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to HLA E -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human HLA E. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- 293 cells, Human spleen tissue lysate and transfected 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88090の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
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| Flow Cyt |
Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. |
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Flow Cyt
Use at an assay dependent concentration. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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関連性
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. -
細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 3133 Human
- Omim: 143010 Human
- SwissProt: P13747 Human
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別名
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
see all
画像
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Flow Cytometry - Anti-HLA E antibody (ab88090)FACS analysis of negative control 293 cells (Black) and HLA E expressing 293 cells (Green) using ab88090.
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Western blot - Anti-HLA E antibody (ab88090)Anti-HLA E antibody (ab88090) at 5 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Western blot - Anti-HLA E antibody (ab88090)All lanes : Anti-HLA E antibody (ab88090) at 5 µg/ml
Lane 1 : 293T cell line (HLA E transfected lysate)
Lane 2 : 293T cell line (Non-transfected lysate)
Lysates/proteins at 50 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab88090 は論文での使用が確認できていません。
