Anti-HLA Class I 抗体 [W6/32] (ab22432)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA Class I antibody [W6/32]
HLA Class I 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [W6/32] to HLA Class I -
由来種
Mouse -
特異性
The antibody recognises HLA-A, HLA-B and HLA-C.
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アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-Frmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human HLA Class I. Purified human tonsil lymphocyte membranes.
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ポジティブ・コントロール
- IHC-Fr: Human heart tissue. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
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特記事項
This product has switched from a hybridoma to recombinant production method on 25th March 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
W6/32 -
ミエローマ
NS1/1-Ag4-1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 0.2 µg/ml.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-Fr |
Use a concentration of 0.05 µg/ml.
|
特記事項 |
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Flow Cyt
Use a concentration of 0.2 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-Fr
Use a concentration of 0.05 µg/ml. |
ターゲット情報
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関連性
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
細胞内局在
Plasma membrane -
参照データベース
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
別名
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
画像
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Immunohistochemical analysis of ab22432 10% paraformaldehyde fixed endothelial cells in frozen Human spleen tissue Human heart tissue labeling HLA Class I with ab22432 at 0.05µg/ml. Detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% PBS-Tween permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HLA Class I with ab22432 at 1µg/mL, blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92494 at 1µg/mL and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% PBS-Tween permeabilized negative cell line K562 labelling HLA Class I with ab22432 at 1µg/mL, blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab227805 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Flow cytometry overlay histogram showing left Jurkat positive cells and right negative K562 cells stained with ab22432 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab22432) (1x106 in 100 μl at 0.2 μg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at for 30 min on ice. Isotype control antibody (black line) was mouse IgG2aκ (ab18413) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab22432 は 35 報の論文で使用されています。
- Xu L et al. Anti-signal recognition particle positive necrotizing myopathy-sjogren's syndrome overlap syndrome: a descriptive study on clinical and myopathology features. BMC Musculoskelet Disord 24:219 (2023). PubMed: 36959614
- Abelin JG et al. Workflow enabling deepscale immunopeptidome, proteome, ubiquitylome, phosphoproteome, and acetylome analyses of sample-limited tissues. Nat Commun 14:1851 (2023). PubMed: 37012232
- Butt N et al. The effect of chemotherapies on the crosstalk interaction between CD8 cytotoxic T-cells and MHC-I peptides in the microenvironment of WHO grade 4 astrocytoma. Folia Neuropathol 61:317-325 (2023). PubMed: 37818692
- Kurdi M et al. The Assessment of Major Histocompatibility Complex (MHC) Class-I Expression in Different Neuromuscular Diseases. Degener Neurol Neuromuscul Dis 11:61-68 (2021). PubMed: 35002356
- Choi J et al. Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations. Cell Rep Methods 1:100084 (2021). PubMed: 35474673