Anti-HLA Class I 抗体 [MEM-147] (ab9090)
Key features and details
- Mouse monoclonal [MEM-147] to HLA Class I
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA Class I antibody [MEM-147]
HLA Class I 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-147] to HLA Class I -
由来種
Mouse -
特異性
Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855). -
アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to HLA Class I. PHA-activated peripheral blood lymphocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-147 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9090の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/100. Use non-reducing conditions.
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特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/100. Use non-reducing conditions. |
ターゲット情報
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関連性
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
細胞内局在
Plasma membrane -
参照データベース
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
別名
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
画像
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Flow cytometric analysis of human leukocytes stained using ab9090 (concentration in sample 1.67 μg/ml, GAM PE, red-filled) from human leukocytes unstained by primary antibody (GAM PE, black-dashed).
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Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)
Secondary
Goat anti-mouse IgG (H+L)-HRP conjugate
Performed under non-reducing conditions. -
Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab9090 は 1 報の論文で使用されています。
- Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454