Anti-HLA Class I A1 抗体 (ab185706)
Key features and details
- Rabbit polyclonal to HLA Class I A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HLA Class I A1 antibody -
製品の詳細
Rabbit polyclonal to HLA Class I A1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human HLA Class I A1 aa 1-300.
Database link: P30443 -
ポジティブ・コントロール
- THP1, HeLa, DU145, SW480 and HepG2 cell extracts; Mouse liver extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185706の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/50 - 1/200.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 41 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 3105 Human
- Omim: 142800 Human
- SwissProt: P30443 Human
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別名
- antigen presenting molecule antibody
- FLJ26655 antibody
- HLA class I histocompatibility antigen, A-1 alpha chain antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human neurilemmoma tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185706. Blue DAPI for nuclear staining.
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All lanes : Anti-HLA Class I A1 antibody (ab185706) at 1/500 dilution
Lane 1 : THP1 cell extract
Lane 2 : HeLa cell extract
Lane 3 : DU145 cell extract
Lane 4 : SW480 cell extract
Lane 5 : HepG2 cell extract
Lane 6 : Mouse liver extract
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185706 は 1 報の論文で使用されています。
- Chen X et al. Decoy receptor-3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease. Exp Ther Med 17:2614-2622 (2019). PubMed: 30906453