Anti-HL60 抗体 [IPO-M6] (ab24093)
Key features and details
- Mouse monoclonal [IPO-M6] to HL60
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HL60 antibody [IPO-M6] -
製品の詳細
Mouse monoclonal [IPO-M6] to HL60 -
由来種
Mouse -
特異性
Reacts with human leukemia cell line HL60 and immuno-precipitates with two proteins with MW of 48 and 52 kDa. ab24093 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.
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アプリケーション
適用あり: IHC-Fr, IP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human HL60.
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ポジティブ・コントロール
- human leukemia cell line HL60
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特記事項
This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative. -
ポリ/モノ
モノクローナル -
クローン名
IPO-M6 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24093の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation. -
別名
- human leukemia cell line HL60 antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab24093 は論文での使用が確認できていません。