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AB228729

Anti-HKDC1 抗体

Anti-HKDC1 antibody

  • JP Deleterious:医薬用外劇物

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(4 Publications)

Rabbit Polyclonal HKDC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Hexokinase HKDC1.

別名を表示する

Hexokinase HKDC1, Hexokinase domain-containing protein 1, HKDC1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HKDC1 antibody (AB228729)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HKDC1 antibody (AB228729)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HKDC1 (green) using ab228729 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)

Paraffin-embedded mouse duodenum tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)

Paraffin-embedded rat brain tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-HKDC1 antibody (AB228729)
  • WB

Supplier Data

Western blot - Anti-HKDC1 antibody (AB228729)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HKDC1 antibody (ab228729) at 1/2000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 103 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human Hexokinase HKDC1. The exact immunogen used to generate this antibody is proprietary information.

Q2TB90

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The HKDC1 protein also known as Hexokinase Domain Containing 1 participates in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate. It has a molecular mass of approximately 102 kDa. This protein is ubiquitously expressed with higher levels in the liver pancreas and certain fetal tissues. HKDC1 plays an important role in maintaining glucose homeostasis particularly under conditions that require enhanced glucose utilization.
Biological function summary

Hexokinase Domain Containing 1 functions as part of the hexokinase enzyme family contributing to the first step of glycolysis. It might not form a complex but shares similar activity with other hexokinase family members supporting cellular energy supply. The presence of HKDC1 in various tissues suggests its involvement in both normal and stress-related metabolic regulation adapting the cell’s energy production to different physiological demands.

Pathways

The activity of HKDC1 is integrated into the glycolytic pathway and indirectly affects the insulin signaling pathway. In glycolysis it links to glucokinase and other hexokinase enzymes playing a part in glucose metabolism and energy generation within the cell. Its activity impacts the homeostatic control of glucose concentration suggesting a regulatory role in maintaining the balance between energy input and output in metabolic pathways.

Mutations or altered expression of HKDC1 could be linked to metabolic syndromes such as diabetes and gestational diabetes mellitus. Aberrant HKDC1 function may disturb glucose homeostasis leading to these conditions. Moreover connection with other proteins like GCK which also participates in glucose phosphorylation highlights its potential importance in metabolic diseases indicating that HKDC1 might be a target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (PubMed : 30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed : 30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity).
See full target information Hexokinase HKDC1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:8034 PubMed37198225

2023

Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina M Pusec,Vladimir Ilievski,Adam De Jesus,Zeenat Farooq,Joseph L Zapater,Nadia Sweis,Hagar Ismail,Md Wasim Khan,Hossein Ardehali,Jose Cordoba-Chacon,Brian T Layden

Endocrinology 163: PubMed35435980

2022

Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph L Zapater,Barton Wicksteed,Brian T Layden

Cancer cell international 20:450 PubMed32943998

2020

HKDC1 promotes the tumorigenesis and glycolysis in lung adenocarcinoma via regulating AMPK/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Wang,Bowen Shi,Yue Zhao,Qijue Lu,Xiang Fei,Chaojing Lu,Chunguang Li,Hezhong Chen

Frontiers in oncology 9:290 PubMed31058090

2019

PGC1β Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Chen,Yang Lv,Ying Sun,Hongyu Zhang,Weiguo Xie,Liyan Zhong,Qi Chen,Min Li,Ling Li,Jia Feng,Athena Yao,Qi Zhang,Xiaodong Huang,Zhendong Yu,Paul Yao
View all publications
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代替製品

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組合せ製品

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Secondary Antibodies

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Primary Antibodies

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Biochemicals

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Primary Antibodies

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