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Anti-HIV2 Nef 抗体 [4B5] (ab22626)

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Key features and details

  • Rat monoclonal [4B5] to HIV2 Nef
  • Suitable for: WB, IP
  • Reacts with: HIV-2
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HIV2 Nef antibody [4B5]

  • 製品の詳細

    Rat monoclonal [4B5] to HIV2 Nef

  • 由来種

    Rat

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: HIV-2

  • 免疫原

    Fusion protein corresponding to HIV2 Nef. Recombinant fusion protein comprising HIV-2 Nef1 sequence fused with E.Coli TrpE protein

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22626の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent dilution.
特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    Nef is a multifunctional protein of 27 kDa in HIV1 and 35 kDa in HIV2. It induces the endocytosis of CD4 Receptor and reduces the MHC1 Receptors. Thereby it enhances viral infectivity and affects T-cell signalling.

  • 細胞内局在

    Cell Membrane

  • 別名

    • F-protein antibody
    • HIV2 nef protein antibody
    • Human immunodeficiency virus 2 nef antibody
    • Nef protein antibody
    • Negative factor antibody
    see all

参考文献 (0)

ab22626 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22626 は論文での使用が確認できていません。

レビューと Q&A

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Western blot abreview for Anti-HIV2 Nef antibody [4B5]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
transfected with HIV2Nef-GFP
Specification
HEK 293T
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 07 2020

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