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リコンビナント

Anti-HIV1 Vif 抗体 [319] (ab66643)

Submit a review Q&A (1)References (10)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [319] to HIV1 Vif
  • Suitable for: ICC/IF, WB
  • Reacts with: Human immunodeficiency virus

製品の概要

  • 製品名

    Anti-HIV1 Vif antibody [319]

  • 製品の詳細

    Mouse monoclonal [319] to HIV1 Vif

  • 由来種

    Mouse

  • 特異性

    This antibody does not cross-react with overexpressed HIV-1 Vif protein from group M subtype K or subtype D.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human immunodeficiency virus

  • 免疫原

    Recombinant full length protein corresponding to HIV1 Vif. Bacterially expressed, hexahistidine amino-terminal tagged HIV1 Vif protein (clade B, HXB-3 isolate).

  • 特記事項

    The production method has been switched from hybridoma to recombinant on 06th June 2025.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    319

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab66643の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/1000. Predicted molecular weight: 23 kDa.
特記事項
ICC/IF
1/100.
WB
1/1000. Predicted molecular weight: 23 kDa.

ターゲット情報

  • 関連性

    HIV1 Vif (viral infectivity factor), supports productive viral replication in non-permissive cells, such as peripheral blood lymphocytes. It functions by inactivating a cellular anti-viral factor named APOBEC3G. Vif binds viral RNA and affects the stability of viral nucleoprotein core. May play a role in viral morphology. Interacts with host ABCE1, which seems to be involved in lentiviruses capsid formation and displays RNase L inhibitor activity. This interaction may play a role in protecting viral RNA from damage during viral assembly.

  • 参照データベース

    • 別名

      • SOR protein antibody
      • Virion infectivity factor antibody

    参考文献 (10)

    ab66643 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab66643 は 10 報の論文で使用されています。

    • Koma T  et al. Involvement of a Rarely Used Splicing SD2b Site in the Regulation of HIV-1 vif mRNA Production as Revealed by a Growth-Adaptive Mutation. Viruses 15:N/A (2023). PubMed: 38140666
    • Cai J  et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. Elife 10:N/A (2021). PubMed: 33835029
    • Koma T  et al. The Expression Level of HIV-1 Vif Is Optimized by Nucleotide Changes in the Genomic SA1D2prox Region during the Viral Adaptation Process. Viruses 13:N/A (2021). PubMed: 34696508
    • Gargan S  et al. HIV-1 Promotes the Degradation of Components of the Type 1 IFN JAK/STAT Pathway and Blocks Anti-viral ISG Induction. EBioMedicine 30:203-216 (2018). WB . PubMed: 29580840
    • Chen H  et al. Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae. Molecules 22:N/A (2017). PubMed: 28885587
    • Desimmie BA  et al. Identification of a tripartite interaction between the N-terminus of HIV-1 Vif and CBFß that is critical for Vif function. Retrovirology 14:19 (2017). PubMed: 28302150
    • Brillen AL  et al. Analysis of Competing HIV-1 Splice Donor Sites Uncovers a Tight Cluster of Splicing Regulatory Elements within Exon 2/2b. J Virol 91:N/A (2017). PubMed: 28446664
    • Nomaguchi M  et al. Production of HIV-1vifmRNA Is Modulated by Natural Nucleotide Variations and SLSA1 RNA Structure in SA1D2prox Genomic Region. Front Microbiol 8:2542 (2017). PubMed: 29326677
    • Nakashima M  et al. Structural Insights into HIV-1 Vif-APOBEC3F Interaction. J Virol 90:1034-47 (2015). PubMed: 26537685
    • Widera M  et al. A functional conserved intronic G run in HIV-1 intron 3 is critical to counteract APOBEC3G-mediated host restriction. Retrovirology 11:72 (2014). WB . PubMed: 25169827

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    I would like to test ab42361 and ab66643 for use in Flow Cytometry

    Read More
    Answer

    For ab42361: DISCOUNT CODE: ***

    For ab66643: DISCOUNT CODE: ***

    Expiration date: August 5th, 2012

    I am very pleased to hear you would like to accept our offer and test ab42361 and ab66643in Flow Cytometry. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    If you routinely perform Flow Cytometry, you may be interested in our EasyLink kits for directly conjugating your primary antibodies:

    https://www.abcam.com/EasyLink

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com