Anti-HIV1 Rev 抗体 [Rev-6] (ab85529)
![Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)](https://www.abcam.co.jp/ps/products/85/ab85529/Images/ab85529-100399-anti-hiv1-rev-antibody-rev-6-western-blot.jpg "Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Rev-6] to HIV1 Rev
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human immunodeficiency virus
製品の概要
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製品名
Anti-HIV1 Rev antibody [Rev-6]
HIV1 Rev 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Rev-6] to HIV1 Rev -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, ELISAmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.
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ポジティブ･コントロール
- WB: HeLa cells transfected with HIV1
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特記事項
The production method has been switched from hybridoma to recombinant on 06th June 2025.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Rev-6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85529の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1.85 µg/ml.
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| WB | (1) |
1/1000. Predicted molecular weight: 13 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1.85 µg/ml. |
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WB
1/1000. Predicted molecular weight: 13 kDa. |
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ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection. -
参照データベース
- Entrez Gene: 155908 Human immunodeficiency virus
- SwissProt: P04618 Human immunodeficiency virus
- SwissProt: P69718 Human immunodeficiency virus
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別名
- Human immunodeficiency virus 1 Rev antibody
- Protein Rev antibody
- Regulator of expression of viral proteins antibody
- Rev antibody
画像
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Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47
Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.
HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.Please note this image was from a different batch produced using hybridoma method
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab85529 は 9 報の論文で使用されています。
- Broussau S et al. Packaging cells for lentiviral vectors generated using the cumate and coumermycin gene induction systems and nanowell single-cell cloning. Mol Ther Methods Clin Dev 29:40-57 (2023). PubMed: 36936448
- Lu W et al. The Polar Region of the HIV-1 Envelope Protein Determines Viral Fusion and Infectivity by Stabilizing the gp120-gp41 Association. J Virol 93:N/A (2019). PubMed: 30651369
- Kyei GB et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). PubMed: 30401776
- Behrens RT et al. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export. J Virol 91:N/A (2017). PubMed: 27852860
- Cochrane A HIV-1 Rev function and RNA nuclear-cytoplasmic export. Methods Mol Biol 1087:103-14 (2014). PubMed: 24158817
- Wong RW et al. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog 9:e1003241 (2013). WB . PubMed: 23555254
- Wong RW et al. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res 41:9471-83 (2013). Human . PubMed: 23945945
- Lund N et al. Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression. Nucleic Acids Res 40:3663-75 (2012). WB . PubMed: 22187150
- Wong R et al. Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy. Retrovirology 8:47 (2011). WB ; Human . PubMed: 21682887