Anti-HIV1 Rev 抗体 [10] (ab25871)
Key features and details
- Mouse monoclonal [10] to HIV1 Rev
- Suitable for: ELISA, WB, Radioimmunoprecipitation
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
製品の概要
-
製品名
Anti-HIV1 Rev antibody [10]
HIV1 Rev 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10] to HIV1 Rev -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WB, Radioimmunoprecipitationmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
To produce this antibody the recombinant Rev (strain BRU) has been used as immunogen. The peptide sequences were not derived from particular strains of HIV1, but were designed as (artificial) B-clade (strain) consensus sequences. Sequences in Swiss prot belong to particular HIV1 strains. For epitope mapping synthetic peptides were used.
-
エピトープ
Epitope mapped to aa34-40 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
10 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/10000.
|
|
WB |
1/500. Predicted molecular weight: 13 kDa.
|
|
Radioimmunoprecipitation |
Use at an assay dependent dilution.
|
特記事項 |
---|
ELISA
1/10000. |
WB
1/500. Predicted molecular weight: 13 kDa. |
Radioimmunoprecipitation
Use at an assay dependent dilution. |
ターゲット情報
-
関連性
HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection. -
参照データベース
- Entrez Gene: 155908 Human immunodeficiency virus
- SwissProt: P04618 Human immunodeficiency virus
- SwissProt: P69718 Human immunodeficiency virus
-
別名
- Human immunodeficiency virus 1 Rev antibody
- Protein Rev antibody
- Regulator of expression of viral proteins antibody
- Rev antibody
画像
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab25871 は 2 報の論文で使用されています。
- Nomaguchi M et al. Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels. J Virol 90:4563-4578 (2016). PubMed: 26912631
- Nomaguchi M et al. Natural single-nucleotide polymorphisms in the 3' region of the hiv-1 pol gene modulate viral replication ability. J Virol 88:4145-60 (2014). WB . PubMed: 24478432