Anti-HIV1 p55 + p24 + p17 抗体 (ab63917)
Key features and details
- Rabbit polyclonal to HIV1 p55 + p24 + p17
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HIV1 p55 + p24 + p17 antibody -
製品の詳細
Rabbit polyclonal to HIV1 p55 + p24 + p17 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.
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ポジティブ･コントロール
- Extract of MT4 cells infected with HIV1 (LAI strain); HeLa whole cell lysate transfected with a plasmid expressing HIV1 gag.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63917の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000. Detects a band of approximately 17,24,55 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/2000. Detects a band of approximately 17,24,55 kDa. |
|
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1. -
参照データベース
- Entrez Gene: 155030 Human immunodeficiency virus
- SwissProt: P05888 Human immunodeficiency virus
- SwissProt: Q70622 Human immunodeficiency virus
- SwissProt: Q79665 Human immunodeficiency virus
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別名
- CA antibody
- Capsid protein p24 antibody
- Gag antibody
see all
画像
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Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1
(LAI strain)
Observed band size: 17,24,55 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)This image is courtesy of an Abreview submitted by David Carpentier.All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with a plasmid expressing HIV1 gag
Lane 2 : Untransfected HeLa whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG (whole molecule) HRP polymer at 1/50000 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab63917 は 38 報の論文で使用されています。
- Segura J et al. HIV-1 release requires Nef-induced caspase activation. PLoS One 18:e0281087 (2023). PubMed: 36780482
- Wang C et al. Antiviral Properties of HIV-1 Capsid Inhibitor GSK878. Antimicrob Agents Chemother 67:e0169422 (2023). PubMed: 37039636
- Shen W et al. Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein. Virol Sin 38:448-458 (2023). PubMed: 37172824
- Alves JM et al. Adjuvant effect of dendritic cells activator Imiquimod in genetic immunization with HIV-1 p55 Gag. J Immunol Tech Infect Dis 12:N/A (2023). PubMed: 37205236
- Gu F et al. HIV-1 promotes ubiquitination of the amyloidogenic C-terminal fragment of APP to support viral replication. Nat Commun 14:4227 (2023). PubMed: 37454116