Anti-HIV1 p24 抗体 [5] (ab63958)
![Western blot - Anti-HIV1 p24 antibody [5] (ab63958)](https://www.abcam.co.jp/ps/products/63/ab63958/Images/ab63958-77227-anti-hiv1-p24-antibody-5-western-blot.jpg "Western blot - Anti-HIV1 p24 antibody [5] (ab63958)")
Key features and details
- Mouse monoclonal [5] to HIV1 p24
- Suitable for: ELISA, WB, ICC/IF, Radioimmunoprecipitation
- Reacts with: Human immunodeficiency virus
- Isotype: IgG2a
製品の概要
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製品名
Anti-HIV1 p24 antibody [5]
HIV1 p24 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5] to HIV1 p24 -
由来種
Mouse -
特異性
Reacts with HIV1 native p24 as well rp24 expressed in bacteria and Cos-7 cells. Reacts with rNef of HIV1 subtype B, C and A. -
アプリケーション
適用あり: ELISA, WB, ICC/IF, Radioimmunoprecipitationmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Synthetic peptide:
TPQDLNTMLNTVGGH
, corresponding to amino acids 48-62 of HIV1 HXB2 p24. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
5 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/25000.
|
|
| WB | (1) |
1/1000. Predicted molecular weight: 24 kDa.
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Radioimmunoprecipitation |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
1/25000. |
|
WB
1/1000. Predicted molecular weight: 24 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Radioimmunoprecipitation
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. -
細胞内局在
Membrane -
参照データベース
- SwissProt: P04585 Human immunodeficiency virus
- SwissProt: P12497 Human immunodeficiency virus
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別名
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
画像
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Western blot - Anti-HIV1 p24 antibody [5] (ab63958)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HIV1 p24 antibody [5] (ab63958) at 1/1000 dilution
All lanes : Hela cells infected with HIV1 (4 hours)
Secondary
All lanes : Goat anti-mouse conjugated to HRP at 1/2500 dilution
Predicted band size: 24 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63958 は 1 報の論文で使用されています。
- Yang X et al. The neutralization of B.1.617.1 and B.1.1.529 sera from convalescent patients and BBIBP-CorV vaccines. iScience 25:105016 (2022). PubMed: 36062074