Anti-HIV1 Nef 抗体 [JR6] (ab42358)
![Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)](https://www.abcam.co.jp/ps/products/42/ab42358/Images/ab42358-172786-anti-hiv1-nef-antibody-jr6-western-blot.jpg "Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)")
Key features and details
- Mouse monoclonal [JR6] to HIV1 Nef
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
製品の概要
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製品名
Anti-HIV1 Nef antibody [JR6]
HIV1 Nef 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [JR6] to HIV1 Nef -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Recombinant Nef protein of HIV1 (B subtype consensus).
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エピトープ
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus) -
特記事項
Also known as clone 01-009.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
IgG fraction, purified by affinty chromatography. -
ポリ/モノ
モノクローナル -
クローン名
JR6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42358の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.2 µg/ml. Predicted molecular weight: 24 kDa.
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| ELISA |
Use a concentration of 0.02 - 1 µg/ml.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.2 µg/ml. Predicted molecular weight: 24 kDa. |
|
ELISA
Use a concentration of 0.02 - 1 µg/ml. Titer in ELISA/WB using rNEF of HIV 1 Subtype A. |
ターゲット情報
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関連性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
参照データベース
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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別名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
画像
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Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
Lane 1 : Recominant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab42358 は 13 報の論文で使用されています。
- Iyer K et al. Identification of 5' upstream sequence involved in HSPBP1 gene transcription and its downregulation during HIV-1 infection. Virus Res 324:199034 (2023). PubMed: 36581045
- Wang Y et al. Nef Suppresses LINE-1 Retrotransposition through Two Distinct Mechanisms. J Virol 96:e0114822 (2022). PubMed: 36197106
- Pushkarsky T et al. Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance. Mol Neurobiol 59:1088-1097 (2022). PubMed: 34843091
- Zhang HG et al. Ubiquitin E3 Ligase c-Cbl Is a Host Negative Regulator of Nef Protein of HIV-1. Front Microbiol 11:597972 (2020). PubMed: 33329486
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674