Anti-HIV1 Nef 抗体 (ab63918)
Key features and details
- Rabbit polyclonal to HIV1 Nef
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus, HIV-2
- Isotype: IgG
製品の概要
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製品名
Anti-HIV1 Nef antibody
HIV1 Nef 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HIV1 Nef -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Human immunodeficiency virus, HIV-2 -
免疫原
Recombinant Nef protein over-expressed in E. coli with a plasmid carrying the Nef coding region of HIV1 virus, subtype B.
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ポジティブ･コントロール
- Extract of MT4 cells uninfected or infected with HIV1 (LAI strain).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
特記事項（精製）
ab63918 is highly purified by several steps of chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63918の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 24 kDa.
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| ELISA |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 24 kDa. |
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ELISA
Use at an assay dependent dilution. |
ターゲット情報
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関連性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
参照データベース
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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別名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
画像
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Western blot - Anti-HIV1 Nef antibody (ab63918)Anti-HIV1 Nef antibody (ab63918) at 1/1000 dilution + Extract of HIV-2 (strain UC2) infected cell
Predicted band size: 24 kDa -
Western blot - Anti-HIV1 Nef antibody (ab63918)All lanes : Anti-HIV1 Nef antibody (ab63918) at 1/1000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63918 は 1 報の論文で使用されています。
- Wang X et al. Role of SIRT1 in HIV-associated kidney disease. Am J Physiol Renal Physiol 319:F335-F344 (2020). PubMed: 32657157