Anti-HIV1 Nef 抗体 [3F2] (ab25866)
Key features and details
- Mouse monoclonal [3F2] to HIV1 Nef
- Suitable for: ELISA, WB, Radioimmunoprecipitation
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
製品の概要
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製品名
Anti-HIV1 Nef antibody [3F2]
HIV1 Nef 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3F2] to HIV1 Nef -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WB, Radioimmunoprecipitationmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Recombinant full length protein
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エピトープ
Mapped to amino acids 31-35 (GAASR, HIV-1 HxB2). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
3F2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25866の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent dilution.
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WB | (1) |
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
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Radioimmunoprecipitation |
Use at an assay dependent dilution.
|
特記事項 |
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ELISA
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa. |
Radioimmunoprecipitation
Use at an assay dependent dilution. |
ターゲット情報
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関連性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
参照データベース
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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別名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab25866 は 6 報の論文で使用されています。
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
- Collings A et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
- Ranki A et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
- Saito Y et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
- Ovod V et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
- Zsolnai B et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091