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Anti-HIV protease 抗体 [1696] (ab8327)

Submit a review Q&A (4)References (8)
Dot Blot - Anti-HIV protease antibody [1696] (ab8327)

    Key features and details

    • Mouse monoclonal [1696] to HIV protease
    • Suitable for: Dot blot
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-HIV protease antibody [1696]
      HIV protease 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [1696] to HIV protease

    • 由来種

      Mouse

    • 特異性

      The antibody recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2). The antibody does not react with the precursor.

    • アプリケーション

      適用あり: Dot blotmore details

    • 免疫原

      Recombinant full length protein corresponding to HIV protease. Bacterially expressed full-length HIV-1 protease.
      Database link: P03366

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab8327の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Dot blot
    Use at an assay dependent concentration.
    特記事項
    Dot blot
    Use at an assay dependent concentration.

    ターゲット情報

    • 関連性

      The HIV1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids in which each monomer contributes each of the 2 catalytic aspartic acids. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. Both the order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV1) infection and AIDS have been shown to suppress the replication of HIV1 and extend the life expectancy of HIV1 infected individuals.

    • 別名

      • HIV-1 protease antibody
      • Human immunodeficiency virus protease antibody
      • PR antibody
      • Retropepsin antibody

    画像

    • Dot Blot - Anti-HIV protease antibody [1696] (ab8327)
      Dot Blot - Anti-HIV protease antibody [1696] (ab8327)

      Dot blot analysis of ab8327. The total amount of ab8327 spotted on the nitrocellulose membrane are indicated in left column.

      Lane 1: ab8327; 0.2 μg/ml

      Lane 2: ab8327; 1.0 μg/ml

      Lane 3: ab8327; 2.0 μg/ml

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (8)

    ab8327 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8327 は 8 報の論文で使用されています。

    • Yu FH  et al. Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity. PLoS One 17:e0262477 (2022). PubMed: 35085286
    • Han S  et al. Continuous progression of hemorrhage of sphenoid ridge meningioma causing cerebral hernia: A case report and literature review. Oncol Lett 20:785-793 (2020). PubMed: 32566005
    • Polyzos AA  et al. Metabolic Reprogramming in Astrocytes Distinguishes Region-Specific Neuronal Susceptibility in Huntington Mice. Cell Metab 29:1258-1273.e11 (2019). PubMed: 30930170
    • Matsunaga S  et al. A cell-free enzymatic activity assay for the evaluation of HIV-1 drug resistance to protease inhibitors. Front Microbiol 6:1220 (2015). WB . PubMed: 26583013
    • Lin PH  et al. Slow immunological progression in HIV-1 CRF07_BC-infected injecting drug users. Emerg Microbes Infect 2:e83 (2013). WB . PubMed: 26038447
    • O'loughlin TL  et al. Diversification and Specialization of HIV Protease Function During In Vitro Evolution. Mol Biol Evol 23:764-72 (2006). WB . PubMed: 16423863
    • M'Barek NB  et al. HIV-2 Protease resistance defined in yeast cells. Retrovirology 3:58 (2006). WB . PubMed: 16956392
    • Lescar J  et al. Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody. Protein Sci 8:2686-96 (1999). PubMed: 10631984

    レビューと Q&A

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    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    . I wanted to know whether I can get Anti-HIV protease antibody of (Rabbit Polyclonal). At present I am using mouse monoclonal HIV-1 protease antibody.Kindly reply me

    Read More
    Answer

    Thank you for contacting us.

    We have anti HIV protease antibodies avialable in catalogue. Please contact our indian distributor for price and shipment;

    Allied Scientific Products
    39/19B Gopal Nagar Road
    Kolkata-700027
    India

    Tel +91 33 24566684/24793109/9039 Freephone 1800 345 7777
    " tabindex="-1"> Email mailto:alliedscientific@vsnl.com Website http://www.alliedscientific.net/

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    can this antibody be provided as Fab fragment only (instead of entire IgG molecule)?
    for crystallography study
    if possible: bulk order OK, how much?

    Read More
    Answer

    Thank you for contacting us.

    I have good news:

    The supplying lab let me know the following:

    We are able to produce Fab fragments from HIV protease antibody [clone1696].
    The minimun amount of the HIV protease must be 5 mg, because there is 10% yield from the purified form. If you are interested in a higher amount of Fab fragments, we would need to increase the input purified material.

    We need to inform the customer that the final product will be in low concentration, lower than 1 mg/ml, the buffer is optional.


    Please let me know if this is something you would be interested in pursuing. If so, you can either reply to myself or send an email directly to our bulk specialist xxx at sales@abcam.com. She will also be able to assist you regarding pricing.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Before ordering your anti-HIV protease Ab, I would like to know at which concentration I could use it as primary Ab in western blotting (I need to recognize the protease of HIV-2).

    Read More
    Answer

    I would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1:500-1:2000) and then adjust the dilution accordingly from the results obtained.

    Read More

    Question

    Dear Sir, Your anti-HIV1 protease Ab recognizes N-terminal of the protease. N-terminal amino acid sequences of my mutant HIV-1 proteases have AQVTLWQPR~ or AQITLWQRP~ instead of PQITLWQRP~ (P1A or/and I3V point mutants). Could you let me know if your antibody reacts with these mutants? Or do you have anit-HIV1 protease Ab to bind to precursor of HIV1 protease (as many of the Ab in papers do)? I will appreciate for your reply. Jongsik Gam

    Read More
    Answer

    This antibody has not been tested against the mutant forms of HIV protease so we do not know whether or not it would react with them.

    Read More

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