AB199739

Anti-Histone H4 (mutated G48D) 抗体 [EP19111]

Anti-Histone H4 (mutated G48D) antibody [EP19111]

RabMAb
Advanced Validation
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(1 Publication)

Rabbit Monoclonal Histone H4 mutated G48D antibody. Suitable for WB, PepArr and reacts with Human samples. Cited in 1 publication.

別名を表示する

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4G48D, H4G48

3 Images

Supplier Data

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

Blocking/Dilution buffer : 5% BSA/TBST.

Exposure times : Lane 1 : 10 seconds; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739) at 1/1000 dilution

Lane 1:

His-tagged human Histone H4 (mutant G48D, aa1-103) recombinant protein, 20 ng

Lane 2:

His-tagged human Histone H4 (WT, aa1-103) recombinant protein, 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Lab

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

Blocking/Diluting buffer and concentration 5% BSA/TBST

All lanes:

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) cytoplasm lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 1s

Peptide Array - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

PepArr

Unknown

Peptide Array - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

All batches of ab199739 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP19111

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, PepArr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "10 µg/mL", "PepArr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 G48D

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 25:3858-3868.e4 PubMed30590054

2018

Engineering an Environment for the Study of Fibrosis: A 3D Human Muscle Model with Endothelium Specificity and Endomysium.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Bersini,Mara Gilardi,Giovanni S Ugolini,Veronica Sansoni,Giuseppe Talò,Silvia Perego,Simona Zanotti,Paola Ostano,Marina Mora,Monica Soncini,Marco Vanoni,Giovanni Lombardi,Matteo Moretti

View all publications

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peptideArrayWebsite

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (mutated G48D) 抗体 [EP19111]

Anti-Histone H4 (mutated G48D) antibody [EP19111]

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

Peptide Array - Anti-Histone H4 (mutated G48D) antibody [EP19111] (AB199739)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise