Anti-Histone H4 (mono methyl K31) 抗体 [EPR17913] (ab177822)
![Western blot - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)](https://www.abcam.co.jp/ps/products/177/ab177822/Images/ab177822-249920-anti-histone-h4-mono-methyl-k31-antibody-epr17913-western-blot.jpg "Western blot - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17913] to Histone H4 (mono methyl K31)
- Suitable for: WB, ICC/IF, PepArr
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Histone H4 (mono methyl K31) antibody [EPR17913]
Histone H4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17913] to Histone H4 (mono methyl K31) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, PepArrmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa whole cell lysate. ICC/IF: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17913 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| ICC/IF |
1/2000.
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| PepArr |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
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ICC/IF
1/2000. |
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PepArr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H4 family. -
翻訳後修飾
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
別名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
画像
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Western blot - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% BSA/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)](https://www.abcam.co.jp/ps/products/177/ab177822/Images/ab177822-249918-anti-histone-h4-mono-methyl-k31-antibody-epr17913-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl K31) with ab177822 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab177822 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution. -
![Peptide Array - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)](https://www.abcam.co.jp/ps/products/177/ab177822/Images/ab177822-261507-anti-histone-h4-mono-methyl-k31-antibody-epr17913-peptide-array.jpg)
Peptide Array - Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)ab177822 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)](https://www.abcam.co.jp/ps/products/177/ab177822/Images/ab177822-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (mono methyl K31) antibody [EPR17913] (ab177822)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab177822 は論文での使用が確認できていません。