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Anti-Histone H4 抗体 [mAbcam 17036] - ChIP Grade (ab17036)

Reviews (4)Q&A (1)References (9)
ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

Key features and details

  • Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade
  • Suitable for: IHC-P, ChIP, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade
    Histone H4 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, ChIP, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Cow, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab13843)

  • ポジティブ･コントロール

    • This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.

  • 特記事項

    To detect histone H4 by ICC/IF, we recommend using ab177840 or ab9052 since these antibodies perform better in this application.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    IgG fraction

  • ポリ/モノ

    モノクローナル

  • クローン名

    mAbcam 17036

  • ミエローマ

    Sp2/0-Ag14

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17036の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use a concentration of 1 µg/ml.
ChIP
Use 5 µg for 25 µg of chromatin.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).Can be blocked with Human Histone H4 peptide (ab13843).

We recommend using 3% milk as the blocking agent for Western blot.
特記事項
IHC-P
Use a concentration of 1 µg/ml.
ChIP
Use 5 µg for 25 µg of chromatin.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).Can be blocked with Human Histone H4 peptide (ab13843).

We recommend using 3% milk as the blocking agent for Western blot.

ターゲット情報

画像

  • ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    IHC image of ab17036 staining in Human Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 2 µg/ml

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein
    Lane 3 : Histone H2B Recombinant Protein
    Lane 4 : Histone H3.1 Recombinant Protein
    Lane 5 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml

    Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
    Lane 2 : HeLa Histone Preparation Nuclear Lysate at 2.5 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

    IHC image of Histone H4 staining in formalin fixed, paraffin embedded human Hodgkin's lymphoma tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein
    Lane 3 : Histone H2B Recombinant Protein
    Lane 4 : Histone H3.1 Recombinant Protein
    Lane 5 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

参考文献 (9)

ab17036 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab17036 は 9 報の論文で使用されています。

  • Radzisheuskaya A  et al. Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis. Mol Cell 81:1749-1765.e8 (2021). PubMed: 33657400
  • Kim SA  et al. Crystal Structure of the LSD1/CoREST Histone Demethylase Bound to Its Nucleosome Substrate. Mol Cell 78:903-914.e4 (2020). PubMed: 32396821
  • van Mierlo G  et al. Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency. Cell Stem Cell 24:123-137.e8 (2019). PubMed: 30472157
  • Hoeijmakers WAM & Bártfai R Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq). Methods Mol Biol 1689:83-101 (2018). PubMed: 29027167
  • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). PubMed: 29670633
  • Ge Y  et al. Mapping dynamic histone modification patterns during arsenic-induced malignant transformation of human bladder cells. Toxicol Appl Pharmacol 355:164-173 (2018). PubMed: 29966674
  • Walsh RM  et al. Phf8 loss confers resistance to depression-like and anxiety-like behaviors in mice. Nat Commun 8:15142 (2017). WB ; Mouse . PubMed: 28485378
  • Dikopoltsev E  et al. FBXO22 protein is required for optimal synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-serine. J Biol Chem 289:33904-15 (2014). PubMed: 25336657
  • Sadhu MJ  et al. Nutritional control of epigenetic processes in yeast and human cells. Genetics 195:831-44 (2013). PubMed: 23979574

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - nuclear (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
25 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Francesca Mcewan

Verified customer

投稿 Oct 11 2023

Immunohistochemistry (Frozen sections) abreview for Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Tumor (colon))
Permeabilization
No
Specification
Tumor (colon)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 30 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - PBS-Tween
Specification
Colon cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 15 2021

Western blot abreview for Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (ES cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
ES cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 22 2018

Question

This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified for diagnostic purposes. Since I did not find any information on this on your website, I wanted to ask you if you have any CE certified antibodies directed against these (human) proteins:

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Answer

All of our products are for research purposes only and are not certified for clinical research applications.

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