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リコンビナントRabMAb

Anti-Histone H4 (acetyl K12) 抗体 [EPR17906] (ab177793)

Reviews (1) Submit a question References (8)
Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Peptide Array - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
  • Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17906] to Histone H4 (acetyl K12)
  • Suitable for: PepArr, Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Histone H4 (acetyl K12) antibody [EPR17906]
    Histone H4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17906] to Histone H4 (acetyl K12)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: PepArr, Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa and NIH/3T3 whole cell lysates treated with 500 ng/ml Trichostatin A for 4 hours. IHC: Human and rat colon ICC/IF: HeLa cells treated with 500 ng/ml Trichostatin A for 4 hours.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR17906

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177793の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
PepArr
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB (1)
1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/2000.
特記事項
PepArr
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/2000.

ターゲット情報

画像

  • Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    All lanes : Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) untreated whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or untreated, labeling Histone H4 (acetyl K12) with ab177793 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line is observed. Acetylation level increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab177793 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177793 at 1/80 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control. 

     

     

  • Peptide Array - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Peptide Array - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    ab177793 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
     
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
     
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
     

  • Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    All lanes : Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793) at 1/10000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) untreated whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (acetyl K12) with ab177793 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H4 (acetyl K12) with ab177793 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Rat colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

参考文献 (8)

ab177793 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab177793 は 8 報の論文で使用されています。

  • Wang YC  et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
  • Xue X  et al. Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming. Clin Transl Med 12:e747 (2022). PubMed: 35220675
  • Xu Y  et al. HDAC1 in the Ovarian Granulosa Cells of Tan Sheep Improves Cumulus Cell Expansion and Oocyte Maturation Independently of the EGF-like Growth Factors. Biology (Basel) 11:N/A (2022). PubMed: 36290368
  • Lin Z & Yuen KWY RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos. Nucleic Acids Res N/A:N/A (2021). PubMed: 33872374
  • Shalini V  et al. Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids. Epigenetics Chromatin 14:3 (2021). PubMed: 33407810
  • Wu Y  et al. Adaptor protein APPL1 links neuronal activity to chromatin remodeling in cultured hippocampal neurons. J Mol Cell Biol 13:335-346 (2021). PubMed: 33104190
  • Yang D  et al. Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation. Cell Death Dis 11:508 (2020). PubMed: 32641713
  • Choo JAMY  et al. The integrated stress response induces R-loops and hinders replication fork progression. Cell Death Dis 11:538 (2020). PubMed: 32678076

レビューと Q&A

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Western blot abreview for Anti-Histone H4 (acetyl K12) antibody [EPR17906]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Platynereis dumerilii Tissue lysate - nuclear (Whole animals: 4 months-old)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
2.5 µg
Treatment
50 nM TSA for 3 days
Specification
Whole animals: 4 months-old
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Quentin Schenkelaars

Verified customer

投稿 Apr 15 2021

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