Anti-Histone H4 (acetyl K12) 抗体 (ab61238)
Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K12)
- Suitable for: ELISA, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human, Drosophila melanogaster, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Histone H4 (acetyl K12) antibody
Histone H4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H4 (acetyl K12) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human, Drosophila melanogaster, African green monkey
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human Histone H4 aa 10-15 (acetyl K12). Synthetic acetylated peptide derived from human Histone H4 around the acetylation site of lysine 12
LGK(Ac)GG
Sequence:LGK(Ac)GG
Database link: P62805 -
ポジティブ･コントロール
- WB: Extracts from COS7 cells, treated with TSA (400nM, 24hours); KB cells, HEK-293 cells (with TSA 400nM 24h). IHC-P: Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-P | (1) |
1/50 - 1/100.
|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H4 family. -
翻訳後修飾
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 318846 Drosophila melanogaster
- Entrez Gene: 3771854 Drosophila melanogaster
- Entrez Gene: 3771893 Drosophila melanogaster
- Entrez Gene: 3771908 Drosophila melanogaster
- Entrez Gene: 3771935 Drosophila melanogaster
- Entrez Gene: 3771938 Drosophila melanogaster
- Entrez Gene: 3771941 Drosophila melanogaster
- Entrez Gene: 3771947 Drosophila melanogaster
see all -
別名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody (ab61238)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
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Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238)All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with no acetylated peptide
Lane 2 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with acetylated peptide
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238)Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution + KB cells
Predicted band size: 11 kDa -
Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238)All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution
Lane 1 : HEK-293 cells (with TSA 400nM 24h)
Lane 2 : HEK-293 cells (without TSA 400nM 24h)
Predicted band size: 11 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab61238 は 23 報の論文で使用されています。
- Huang R et al. HDAC11 inhibition disrupts porcine oocyte meiosis via regulating a-tubulin acetylation and histone modifications. Aging (Albany NY) 13:8849-8864 (2021). PubMed: 33742608
- Díaz-Godínez C et al. New Insights on NETosis Induced by Entamoeba histolytica: Dependence on ROS from Amoebas and Extracellular MPO Activity. Antioxidants (Basel) 10:N/A (2021). PubMed: 34206992
- Viana Borges J et al. Social isolation and social support at adulthood affect epigenetic mechanisms, brain-derived neurotrophic factor levels and behavior of chronically stressed rats. Behav Brain Res 366:36-44 (2019). PubMed: 30880220
- Campos-Fernández E et al. Prognostic value of histone H3 serine 10 phosphorylation and histone H4 lysine 12 acetylation in oral squamous cell carcinoma. Histopathology 74:227-238 (2019). PubMed: 30007038
- Jarmasz JS et al. Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure. Alcohol Clin Exp Res 43:1145-1162 (2019). PubMed: 31074890