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AB272144

Anti-Histone H3 (tri methyl K4, phospho T6) 抗体

Anti-Histone H3 (tri methyl K4, phospho T6) antibody

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Rabbit Polyclonal H3 tri methyl K4 + phospho T6 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Immunofluoescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K4, phospho T6) with ab272144 at a 1/50 dilution for 1 hour at room temperature in ICC/IF (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

ChIP of Histone H3 (tri methyl K4, phospho T6). Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2 μg of ab272144 to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)
  • WB

Unknown

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparations at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)
  • WB

Unknown

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Dot Blot analysis of Histone H3 (tri methyl K4, phospho T6) labeled with ab272144 at 1/1000 dilution.

Lane 1 : Unmodified.

Lane 2 : pT6.

Lane 3 : K4Me1pT6.

Lane 4 : K4Me2pT6.

Lane 5 : K4Me3pT6.

Load : 3, 1, and 0.3 picomoles of peptide.

Block : 5% BLOTTO overnight at 4°C.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Caenorhabditis elegans

アプリケーション

Dot, WB, ICC/IF, ChIP

applications

免疫原

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

特異性

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Anti-Histone H3 [Trimethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 tri methyl K4 + phospho T6
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組合せ製品

Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB4174

Anti-Histone H2A.Z antibody - ChIP Grade

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174)

  • 4
  • 31

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