AB9050

Anti-Histone H3 (tri methyl K36) 抗体 - ChIP Grade

Name: Anti-Histone H3 (tri methyl K36) 抗体 - ChIP Grade (ab9050) | Abcam
Brand: Abcam Limited
SKU: AB9050
Rating: 5 (64 reviews)

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

(64 Reviews)

(975 Publications)

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, ICC/IF, ChIP. Suitable for Cow, Human.

- Over 970 publications
- Trusted since 2004

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me3, H3K36me, H3K36

5 Images

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ChIP

Lab

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab9050 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active).

Schematic diagram of the γ-Actin gene is shown on the top of the figure.

Black boxes represent exons and thin lines represent introns.

PCR products are depicted as bars under the gene.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ICC/IF

AbReview29364****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri methyl K36) in Saos-2 (Human osteosarcoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/1000) for 1 hour. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri-methyl K36) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed with 100% methanol for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab9050 at 0.1 μg/ml and ab7291 (anti beta-Tubulin) at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a goat anti-rabbit AlexaFluor^®488 secondary (ab150081) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor^®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

Negative controls : 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 stained in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

Cells were fixed with 100% methanol for 5 minutes at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9050 at 0.1 μg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) (pseudo-colored red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) secondary antibody (colored green) used at 1 μg/ml for 1 hour at room temperature.

DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1 hour at room temperature.

Project

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) at 1 µg/mL

Lanes 1, 4 and 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - unmodified K36

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K37) peptide (<a href='/products/unavailable/human-histone-h3-tri-methyl-k37-peptide-ab24417'>ab24417</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Cow, Human

アプリケーション

WB, ICC/IF, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1-1 µg/mL", "ICCIF-species-notes": "We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-alexa-fluor-488-preadsorbed-ab150081'>ab150081</a>) secondary antibody." }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Plants": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rice": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Silk worm": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Trypanosoma brucei": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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下記製品もご検討ください

AB282572

Anti-Histone H3 (tri methyl K36) antibody [EPR23525-232] - ChIP Grade

1 Reviews

製品ページを見る

こちらの製品も推奨する理由

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製品の詳細

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, WB in cow, human, saccharomyces cerevisiae samples.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has been cited over 975 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has 64 independent reviews from customers.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) specifically detects Histone H3 Tri Methyl-K36 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 µg selling sizes.

H3K36me3 is a crucial epigenetic modification that involves the tri-methylation of lysine 36 on Histone H3, playing a significant role in gene regulation and DNA repair. Utilizing our tri methyl K36 antibody can significantly enhance the detection and study of this modification. Research indicates that H3K36me3 is associated with various cancers, making it a vital target for understanding disease mechanisms and developing new therapeutic strategies. Abnormal H3K36me3 levels are linked to cancer progression and poor prognosis. Studying H3K36me3 modifications can also provide insights into potential therapeutic targets for conditions like congenital heart defects and neurological disorders.

For detection of methylated histone H3.

Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 tri methyl K36

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文献 (975)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8238 PubMed38086830

2023

Epigenetic modifications regulate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Zhiyuan Jin,Fa Cui,Long Zhao,Xiaoyu Zhang,Jinchao Chen,Jing Zhang,Yanyan Li,Yongpeng Li,Yanxiao Niu,Wenli Zhang,Caixia Gao,Xiangdong Fu,Yiping Tong,Lei Wang,Hong-Qing Ling,Junming Li,Jun Xiao

PLoS biology 21:e3002417 PubMed38048343

2023

FMRP deficiency leads to multifactorial dysregulation of splicing and mislocalization of MBNL1 to the cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Suna Jung,Sneha Shah,Geongoo Han,Joel D Richter

Communications biology 6:1225 PubMed38044380

2023

Organ-specific characteristics govern the relationship between histone code dynamics and transcriptional reprogramming during nitrogen response in tomato.

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Species

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Russell Julian,Ryan M Patrick,Ying Li

Life science alliance 7: PubMed38011998

2023

2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibition.

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Unspecified application

Species

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Marios Gavriil,Marco Proietto,Nicole Paczia,Aurelien Ginolhac,Rashi Halder,Elena Valceschini,Thomas Sauter,Carole L Linster,Lasse Sinkkonen

Scientific reports 13:19885 PubMed37963940

2023

SETD5 regulates the OGT-catalyzed O-GlcNAcylation of RNA polymerase II, which is involved in the stemness of colorectal cancer cells.

Applications

Unspecified application

Species

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Hye In Cho,Sora Jo,Min Seong Kim,Han Byeol Kim,Xingzhe Liu,Yanhua Xuan,Jin Won Cho,Yeun Kyu Jang

Clinical and translational medicine 13:e1475 PubMed37962020

2023

Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway.

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Species

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Chunxiao Ma,Min Liu,Wenxin Feng,Hanyu Rao,Wei Zhang,Changwei Liu,Yue Xu,Ziyi Wang,Yan Teng,Xiao Yang,Li Ni,Jin Xu,Wei-Qiang Gao,Bing Lu,Li Li

Life science alliance 7: PubMed37940188

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DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection.

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Neysha Tirado-Class,Caitlin Hathaway,Anthony Nelligan,Thuan Nguyen,Huzefa Dungrawala

The New phytologist 241:267-282 PubMed37849024

2023

R-loops act as regulatory switches modulating transcription of COLD-responsive genes in rice.

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Species

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Zexue He,Mengqi Li,Xiucai Pan,Yulian Peng,Yining Shi,Qi Han,Manli Shi,Linwei She,Gennadii Borovskii,Xiaojun Chen,Xiaofeng Gu,Xuejiao Cheng,Wenli Zhang

Science advances 9:eadg5109 PubMed37831776

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Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription.

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Species

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Donald P Cameron,Jan Grosser,Swetlana Ladigan,Vladislav Kuzin,Evanthia Iliopoulou,Anika Wiegard,Hajar Benredjem,Kathryn Jackson,Sven T Liffers,Smiths Lueong,Phyllis F Cheung,Deepak Vangala,Michael Pohl,Richard Viebahn,Christian Teschendorf,Heiner Wolters,Selami Usta,Keyi Geng,Claudia Kutter,Marie Arsenian-Henriksson,Jens T Siveke,Andrea Tannapfel,Wolff Schmiegel,Stephan A Hahn,Laura Baranello

Nature 622:619-626 PubMed37758950

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Inhibition of fatty acid oxidation enables heart regeneration in adult mice.

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Xiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas Braun

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Anti-Histone H3 (tri methyl K36) 抗体 - ChIP Grade

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

下記製品もご検討ください

Anti-Histone H3 (tri methyl K36) antibody [EPR23525-232] - ChIP Grade

こちらの製品も推奨する理由

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (975)

Abcam product promise

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