AB14955

Anti-Histone H3 (phospho S10) 抗体 [mAbcam 14955]

Name: Anti-Histone H3 (phospho S10) 抗体 [mAbcam 14955] (ab14955) | Abcam
Brand: Abcam Limited
SKU: AB14955
Price: 79000 JPY
Availability: InStock
Rating: 4 (25 reviews)

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]

BOND RX™ Validated
詳細を見る

(25 Reviews)

(273 Publications)

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, ELISA. Suitable for Drosophila melanogaster, Human, Mouse.

- Over 230 publications
- Trusted since 2005

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

IHC image of ab14955 staining Histone H3 (phospho S10) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14955, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

This data was developed using the same antibody clone in a different buffer formulation containing PBS only (ab238673).

ab238673 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab238673 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

ab14955 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab14955 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Flow Cytometry (Intracellular) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Flow cytometry dot plot showing HeLa cells stained with ab14955 (red line). The cells were fixed with 100% ethanol. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab14955) (1x 10^6 in 100μl at 1μg/ml (1/500)) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 for 30 min at 22°C. DNA staining was performed by pre-incubation with RNase A (250μg/ml) followed by DAPI.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Flow Cyt (Intra)

Collaborator

Flow Cytometry (Intracellular) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

ab14955 (1/1000) stainning a population of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells positive for Histone H3 (phospho S10). Cells were trypsizined, pelleted and fixed in ice cold ethanol. Cellular debris was eliminated and the FL2-A/FL2-W was used to eliminate clumping cells. For further experimental details please refer to abreview.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 1 µg/mL

Lanes 1, 5 and 7:

Control HeLa Histone Prep at 0.5 µg

Lanes 2, 6 and 8:

Colcemid treated HeLa Histone prep at 0.5 µg

Lane 3:

Control HeLa Histone Prep at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644)

Lane 4:

Colcemid treated HeLa Histone prep at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644)

Lane 9:

Control HeLa Histone Prep at 0.5 µg with Human Histone H3 (phospho S28) peptide (ab14793)

Lane 10:

Colcemid treated HeLa Histone prep at 0.5 µg with Human Histone H3 (phospho S28) peptide (ab14793)

Secondary

All lanes:

Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Project

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 0.5 µg/mL

Lanes 1, 3 and 7:

Control HeLa Histone prep at 5 µg

Lanes 2, 4 and 8:

Colecemid treated HeLa Histone prep at 5 µg

Lane 5:

Control HeLa Histone prep at 5 µg with Human Histone H3 (phospho S28) peptide (ab14793)

Lane 6:

Colecemid treated HeLa Histone prep at 5 µg with Human Histone H3 (phospho S28) peptide (ab14793)

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 20 kDa

false

Lab

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 1/2000 dilution

Lane 1:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with calyculin A at 10 µg

Lane 3:

HeLa cell lysate treated with calyculin A and alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/20000 dilution

Predicted band size: 15 kDa

false

Exposure time: 1s

Collaborator6731210****

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Blocking : 10% BSA

wee shRNA embryos (lane 2) should display elevated phospho H3Ser10 levels relative to wild type

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 1/1000 dilution

Lane 1:

Wild type 0-4 hour old fruit fly embryo lysate

Lane 2:

0-4 hour old fruit fly embryo lysate expressing wee RNAi

Secondary

All lanes:

Anti-mouse IgG, peroxidase-linked at 1/10000 dilution

Predicted band size: 15 kDa

true

Exposure time: 5s

Image courtesy of Richelle Sopko, Harvard University, U.S.A

ELISA - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

ELISA

Project666****

ELISA - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

By ELISA, ab14955 detects :
the singly modified phospho S10 peptide and the dual modified phospho S10 and tri methyl K9 peptide (the orange and grey lines respectively),
less strongly detects the phospho S28 peptide (purple line),
does not detect the equivalent non-modified Histone H3 peptide for S10 or the singly modified tri methyl K9 peptide (the 2 lines at the bottom of the figure).

The antibody recognises phospho S28 by ELISA (although a phospho S28 peptide does not block the antibody in Western blotting).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

mAbcam 14955

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Human, Drosophila melanogaster

アプリケーション

IHC-P, ELISA, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab14955 recognises phospho S10 on Histone H3. It recognises a phospho S28 peptide by ELISA, but is not blocked by phospho S28 in WB.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Arabidopsis thaliana": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Aspergillus nidulans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Caenorhabditis elegans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chlamydomonas reinhardtii": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Indian muntjac": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Neurospora crassa": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Oncopeltus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Saccharomyces cerevisiae": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Schizosaccharomyces pombe": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Tobacco": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Drosophila melanogaster, Human, Mouse samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (phospho S10) [mAbcam 14955] (ab14955) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (phospho S10) [mAbcam 14955] (ab14955) was first used in a scientific publication in 2005 and has been cited over 230 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (phospho S10) [mAbcam 14955] (ab14955) has over 20 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [mAbcam 14955] also available for your convenience: ab14955, Alexa Fluor® 647 - ab196698, Alexa Fluor® 488 - ab197502, Carrier free - ab238673

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 phospho S10

style

left-column

文献 (273)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011656 PubMed40911633

2025

Distinct cellular and reproductive consequences of meiotic chromosome synapsis defects in syce2 and sycp1 mutant zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Olaya,Ilara N Yilmaz,Naima Nour-Kasally,Ross E Charboneau,Bruce W Draper,Sean M Burgess

eLife 13: PubMed40698928

2025

Identification of novel human microcephaly-linked protein that mediates cortical progenitor cell division and corticogenesis through .

Applications

Unspecified application

Species

Unspecified reactive species

Aurelie Carabalona,Henna Kallo,Maryanne Gonzalez,Liliia Andriichuk,Ellinoora Elomaa,Florence Molinari,Christiana Fragkou,Pekka Lappalainen,Marja W Wessels,Juha Saarikangas,Claudio Rivera

Genome research 35:1733-1744 PubMed40480831

2025

Mitotic chromosomes harbor cell type- and species-specific structural features within a universal loop array conformation.

Applications

Unspecified application

Species

Unspecified reactive species

Marlies E Oomen,A Nicole Fox,Inma Gonzalez,Amandine Molliex,Thaleia Papadopoulou,Pablo Navarro,Job Dekker

Cancer cell international 25:160 PubMed40264142

2025

Taurochenodeoxycholic acid suppresses the progression of glioblastoma via HMGCS1/HMGCR/GPX4 signaling pathway in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiumin Xue,Ziwan He,Furui Liu,Qian Wang,Zhichao Chen,Lin Lin,Danni Chen,Yinfeng Yuan,Zhihui Huang,Yongjie Wang

Cell death & disease 16:250 PubMed40185715

2025

Generation and validation of a novel multitarget small molecule in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aizpea Artetxe-Zurutuza,Nerea Iturrioz-Rodriguez,Joseba Elizazu,Mireia Toledano-Pinedo,Alicia Porro-Pérez,Irati De Goñi,Alejandro Elua-Pinin,Linda Schäker-Hübner,Mikel Azkargorta,Felix Elortza,Isabel Iriepa,Francisco Lòpez-Muñoz,Veronica Moncho-Amor,Finn K Hansen,Nicolás Sampron,Jose Luis Marco-Contelles,Ander Matheu

Molecular medicine (Cambridge, Mass.) 31:13 PubMed39819316

2025

Nootkatone inhibits the progression of glioblastoma by activating the ATF4-CHOP-CHAC1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Xiumin Xue,Zhichao Chen,Wei Zhang,Yiming Qian,Danni Chen,Lin Lin,Yinfeng Yuan,Weiqiao Zhao,Zhihui Huang,Yongjie Wang

Nature communications 16:746 PubMed39820273

2025

Mitotic chromatin marking governs the segregation of DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Juliette Ferrand,Juliette Dabin,Odile Chevallier,Matteo Kane-Charvin,Ariana Kupai,Joel Hrit,Scott B Rothbart,Sophie E Polo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409086 PubMed39804990

2025

TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hua,Lingting Jin,Zheyu Fang,Yiyun Weng,Yuan Zhang,Jingjing Zhang,Dewei Xie,Yang Tang,Siyu Guo,Yingying Huang,Yilin Dai,Jia Li,Zhihui Huang,Xu Zhang

Nucleic acids research 53: PubMed39704103

2024

The interplay of the translocase activity and protein recruitment function of PICH in ultrafine anaphase bridge resolution and genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Kong,Kun Chen,Primrose Chanboonyasitt,Huadong Jiang,Ka Yan Wong,Hoi Tang Ma,Ying Wai Chan

Cells 13: PubMed39682688

2024

A Novel Class of FKBP12 Ligands Rescues Premature Aging Phenotypes Associated with Myotonic Dystrophy Type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Mikel García-Puga,Gorka Gerenu,Ariadna Bargiela,Jorge Espinosa-Espinosa,Laura Mosqueira-Martín,Maialen Sagartzazu-Aizpurua,Jesús M Aizpurua,Ainara Vallejo-Illarramendi,Rubén Artero,Adolfo López de Munain,Ander Matheu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (phospho S10) 抗体 [mAbcam 14955]

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Flow Cytometry (Intracellular) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Flow Cytometry (Intracellular) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

ELISA - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (273)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise