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AB7766

Anti-Histone H3 (di methyl K4) 抗体 - ChIP Grade

Anti-Histone H3 (di methyl K4) antibody - ChIP Grade

5

(25 Reviews)

|

(352 Publications)

Rabbit Polyclonal H3 di methyl K4 antibody. Suitable for ICC/IF, ChIP, WB, PepArr and reacts with Human, Mouse, Rat, Cow, Synthetic peptide - Human samples. Cited in 352 publications.

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4

8 Images
Functional Studies - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • FuncS

Unknown

Functional Studies - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

MCF7 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of tranylcypromine hydrochloride (ab120606). Increased expression of Histone 3 K4 di-methyl (ab7766) in MCF7 cells correlates with an increase in tranylcypromine hydrochloride concentration, as described in literature.

Nuclear extracts were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7766 at 1 μg /ml and ab1791 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualized using ECL development solution.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • ICC/IF

AbReview6832****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

ab7766 at 1/200 staining human U2OS (osteosarcoma) cells by ICC/IF. The cells were paraformaldehyde fixed and then stained with the antibody for 1 hour. A Cy2 ® conjugated donkey anti-rabbit antibody was used as the secondary (green). The image shows uniformal staining of the whole nucleus, with several specles found. The insert shows H3-di methyl K4 of tw2 cells only. DAPI nuclear staining is shown in blue.

This image is courtesy of an Abreview submitted by Dr Alexander Rapp

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

ab7766 staining Histone H3 (di methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7766 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ChIP - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab7766 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • WB

Unknown

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

Blocking buffer : 2% BSA

Gel type : MES

Exposure Time : 1 minute

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL

Lane 1:

HeLa nuclear lysate (triton enriched) at 20 µg

Lane 2:

NIH 3T3 nuclear lysate (triton enriched) at 20 µg

Lane 3:

PC12 nuclear lysate (triton enriched at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

Dimethylated lysine 4 (green) is found in several hundred small nuclear foci that do not colocalize with condensed regions of chromatin (DAPI stained, red). The perinuclear and perinucleolar heterochromatin do not stain with this antibody.

This image is courtesy of Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • WB

Unknown

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 14 kDa,17 kDa

true

Exposure time: 4min

Peptide Array - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
  • PepArr

Unknown

Peptide Array - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)

All batches of ab7766 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - di methyl K4 peptide (ab7768), indicating that this antibody specifically recognises the Histone H3 - di methyl K4 modification.

  1. ab1340 - Histone H3 - mono methyl K4
  2. ab1342 - Histone H3 - tri methyl K4
  3. ab1771 - Histone H3 - mono methyl K9
  4. ab1772 - Histone H3 - di methyl K9
  5. ab1773 - Histone H3 - tri methyl K9
  6. ab1780 - Histone H3 - mono methyl K27
  7. ab1781 - Histone H3 - di methyl K27
  8. ab1782 - Histone H3 - tri methyl K27
  9. ab7228 - Histone H3 - unmodified
  10. ab7768 - Histone H3 - di methyl K4

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Cow, Human

アプリケーション

ICC/IF, PepArr, WB, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab176878.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "<p>Use ALDOA ChIP primer pair <a href='/products/reagents/aldoa-positive-control-chip-primer-pair-ab269260'>ab269260</a> as positive control.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Candida albicans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Common marmoset": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Cow": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Plasmodium falciparum": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Saccharomyces cerevisiae": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Schizosaccharomyces pombe": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Synthetic peptide - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "0.2-2 µg/mL", "PepArr-species-notes": "<p></p>" }, "Tetrahymena": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }

製品の詳細

For detection of Histone H3 specifically methylated at position Lys 4. This antibody was used in a screen by Dover et al, (2002) to isolate yeast mutants that are unable to methylate Lysine 4. In immunofluorescence, this antibody detects foci in the nucleus that are non-colocalising with condensed chromatin. The perinuclear and perinucleolar heterochromatin are not stained with this antibody.

Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 di methyl K4

文献 (352)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8716 PubMed41028718

2025

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Noberini,Giulia Robusti,Alessandro Vai,Evelyn Oliva Savoia,Maria Giovanna Jodice,Giovanni Bertalot,Betül Çat,Isabella Pallavicini,Giuseppina Bonizzi,Maria Capra,Claudia Anna Sangalli,Federico Zambelli,Nicola Fusco,Salvatore Pece,Giulio Pavesi,Saverio Minucci,Tiziana Bonaldi

Epigenetics & chromatin 18:62 PubMed41013562

2025

Antibiotics induce overexpression of alpha satellite DNA accompanied with epigenetic changes at alpha satellite arrays as well as genome-wide.

Applications

Unspecified application

Species

Unspecified reactive species

Sven Ljubić,Maja Matulić,Damir Đermić,Maria Chiara Feliciello,Alfredo Procino,Đurđica Ugarković,Isidoro Feliciello

International journal of molecular sciences 26: PubMed40869273

2025

and the -COMPASS-like Complex Regulate Cardiac Progenitor Cell Division in the Embryonic Heart Tube.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Farmer,Mark H Inlow,Shaad M Ahmad,Kristopher R Schwab

PLoS genetics 21:e1011689 PubMed40315272

2025

Centromere-size reduction and chromatin state dynamics following intergenomic hybridization in cotton.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlei Han,Guanjing Hu,Yan Dai,Xin Zhang,Jingjing Tian,Jialiang Zhou,Xinqi Xu,Qi Chen,Xiaobing Kou,Lei Xu,Xinyu Wu,Ziying Sun,Jiahui Geng,Lin Li,Chenyu Qiu,Teame Gereziher Mehari,Baohua Wang,Hui Zhang,Xinlian Shen,Zhenzhen Xu,Jonathan F Wendel,Kai Wang

Clinical epigenetics 17:20 PubMed39920865

2025

Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Chen,Haibo Zhu,Fucheng Guo,Luyao Wang,Wenli Zhang,Ruizhi Liu,Xiaoling Zhang,Xiangpeng Dai

Cell reports 44:115272 PubMed39908143

2025

KMT2C/KMT2D-dependent H3K4me1 mediates changes in DNA replication timing and origin activity during a cell fate transition.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Gökbuget,Liana Goehring,Ryan M Boileau,Kayla Lenshoek,Tony T Huang,Robert Blelloch

Cell reports 44:115213 PubMed39817906

2025

Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Robert S Porter,Sojin An,Maria C Gavilan,Masayoshi Nagai,Yumie Murata-Nakamura,Bo Zhou,Katherine M Bonefas,Olivier Dionne,Jeru Manoj Manuel,Joannie St-Germain,Suzanne Gascon,Jacqueline Kim,Liam Browning,Benoit Laurent,Uhn-Soo Cho,Shigeki Iwase

Nature 637:974-982 PubMed39779849

2025

Bidirectional histone monoaminylation dynamics regulate neural rhythmicity.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfei Zheng,Benjamin H Weekley,David A Vinson,Shuai Zhao,Ryan M Bastle,Robert E Thompson,Stephanie Stransky,Aarthi Ramakrishnan,Ashley M Cunningham,Sohini Dutta,Jennifer C Chan,Giuseppina Di Salvo,Min Chen,Nan Zhang,Jinghua Wu,Sasha L Fulton,Lingchun Kong,Haifeng Wang,Baichao Zhang,Lauren Vostal,Akhil Upad,Lauren Dierdorff,Li Shen,Henrik Molina,Simone Sidoli,Tom W Muir,Haitao Li,Yael David,Ian Maze

Nucleic acids research 53: PubMed39698834

2024

PRMT1-mediated methylation regulates MLL2 stability and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dongju An,Jihyun Kim,Byul Moon,Hyoungmin Kim,Hoa Nguyen,Sunghu Park,J Eugene Lee,Jung-Ae Kim,Jaehoon Kim

Cardiovascular toxicology 25:34-47 PubMed39521734

2024

Isoprenaline Inhibits Histone Demethylase LSD1 to Induce Cardiac Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Wu,Bo Yang,Yingze Sun,Guanwei Fan,Lina Ma,Ying Ma,Xianjia Xiong,Hui Zhou,Huiping Wang,Ling Zhang,Bing Yang
View all publications

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当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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