Anti-Histone H3 (di methyl K4) 抗体 - ChIP Grade
Anti-Histone H3 (di methyl K4) antibody - ChIP Grade
5
(25 Reviews)
|
(352 Publications)
Rabbit Polyclonal H3 di methyl K4 antibody. Suitable for ICC/IF, ChIP, WB, PepArr and reacts with Human, Mouse, Rat, Cow, Synthetic peptide - Human samples. Cited in 352 publications.
別名を表示する
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4
- FuncS
Unknown
Functional Studies - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
MCF7 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of tranylcypromine hydrochloride (ab120606). Increased expression of Histone 3 K4 di-methyl (ab7766) in MCF7 cells correlates with an increase in tranylcypromine hydrochloride concentration, as described in literature.
Nuclear extracts were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7766 at 1 μg /ml and ab1791 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualized using ECL development solution.
- ICC/IF
AbReview6832****
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
ab7766 at 1/200 staining human U2OS (osteosarcoma) cells by ICC/IF. The cells were paraformaldehyde fixed and then stained with the antibody for 1 hour. A Cy2 ® conjugated donkey anti-rabbit antibody was used as the secondary (green). The image shows uniformal staining of the whole nucleus, with several specles found. The insert shows H3-di methyl K4 of tw2 cells only. DAPI nuclear staining is shown in blue.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
ab7766 staining Histone H3 (di methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7766 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ChIP
Unknown
ChIP - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab7766 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
- WB
Unknown
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Blocking buffer : 2% BSA
Gel type : MES
Exposure Time : 1 minute
All lanes:
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL
Lane 1:
HeLa nuclear lysate (triton enriched) at 20 µg
Lane 2:
NIH 3T3 nuclear lysate (triton enriched) at 20 µg
Lane 3:
PC12 nuclear lysate (triton enriched at 20 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
false
- ICC/IF
Collaborator
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Dimethylated lysine 4 (green) is found in several hundred small nuclear foci that do not colocalize with condensed regions of chromatin (DAPI stained, red). The perinuclear and perinucleolar heterochromatin do not stain with this antibody.
This image is courtesy of Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
- WB
Unknown
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
All lanes:
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL
All lanes:
Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa,17 kDa
true
Exposure time: 4min
- PepArr
Unknown
Peptide Array - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
All batches of ab7766 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - di methyl K4 peptide (ab7768), indicating that this antibody specifically recognises the Histone H3 - di methyl K4 modification.
- ab1340 - Histone H3 - mono methyl K4
- ab1342 - Histone H3 - tri methyl K4
- ab1771 - Histone H3 - mono methyl K9
- ab1772 - Histone H3 - di methyl K9
- ab1773 - Histone H3 - tri methyl K9
- ab1780 - Histone H3 - mono methyl K27
- ab1781 - Histone H3 - di methyl K27
- ab1782 - Histone H3 - tri methyl K27
- ab7228 - Histone H3 - unmodified
- ab7768 - Histone H3 - di methyl K4
Reactivity data
製品の詳細
For detection of Histone H3 specifically methylated at position Lys 4. This antibody was used in a screen by Dover et al, (2002) to isolate yeast mutants that are unable to methylate Lysine 4. In immunofluorescence, this antibody detects foci in the nucleus that are non-colocalising with condensed chromatin. The perinuclear and perinucleolar heterochromatin are not stained with this antibody.
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
製品プロトコール
- Visit the General protocols
- Visit the Troubleshooting
ターゲットの情報
文献 (352)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:8716 PubMed41028718
2025
Applications
Unspecified application
Species
Unspecified reactive species
Epigenetics & chromatin 18:62 PubMed41013562
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 26: PubMed40869273
2025
Applications
Unspecified application
Species
Unspecified reactive species
PLoS genetics 21:e1011689 PubMed40315272
2025
Applications
Unspecified application
Species
Unspecified reactive species
Clinical epigenetics 17:20 PubMed39920865
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 44:115272 PubMed39908143
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 44:115213 PubMed39817906
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature 637:974-982 PubMed39779849
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nucleic acids research 53: PubMed39698834
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cardiovascular toxicology 25:34-47 PubMed39521734
2024
Applications
Unspecified application
Species
Unspecified reactive species
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com