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AB1791

Anti-Histone H3 抗体 - Nuclear Marker and ChIP Grade

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

5

(233 Reviews)

|

(5687 Publications)

Anti-Histone H3 antibody - ChIP Grade Nuclear Marker (ab1791) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Human, Mouse, Rat, Arabidopsis, Drosophila, Muntjac, S.cerevisiae, S.pombe, Xenopus.

- Over 4740 publications
- Trusted since 2002

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

14 Images
ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • ChIP

Unknown

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab1791 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • IHC-P

AbReview43801****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1791 staining Histone H3 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA + 1% FBS) for 16 hours. An un-diluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • ICC/IF

AbReview20971****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1791 staining Histone H3 in HeLa (Human epithelial cell line from cervix adenocarcinoma) by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with the primary antibody (1/300 in PBS + 0.2% gelatin) for 20 minutes at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

Green - Histone H3.
Blue - DAPI.
Red - Tubulin.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1791 staining Histone H3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1791 at 0.1 μg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Immunoprecipitation - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • IP

Lab

Immunoprecipitation - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Histone H3 - ChIP Grade was immunoprecipitated using 0.5mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract, 5 μg of Rabbit polyclonal to and 50 μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10 minutes, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

Proteins were eluted by addition of 40 μl SDS loading buffer and incubated for 10 minutes at 70°C; 10 μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1791.

Secondary Antibody : Mouse anti-rabbit HRP light chain (HRP) (ab99697).

Band : 15kDa; Histone H3 - ChIP Grade

All lanes:

Immunoprecipitation - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791)

Predicted band size: 15 kDa

true

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • IHC-P

AbReview42146****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1796 staining Histone H3 in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde permeabilized with 0.05% Triton X-100 in PBS for 30 minutes and blocked with 5% BSA for 1 hour; antigen retrieval was by heat mediation in sodium citrate pH 6. Samples were incubated with the primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400 dilution) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1791 staining Histone H3 (red) in rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections).

Tissue was fixed with formaldehyde permeabilized with 0.1% TBS-TritonX and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with the primary antibody (1/500 in 10% normal goat serum) for 24 hours at 24°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/500 dilution) was used as the secondary antibody.

Green - Nucleus staining.
Red - Histone H3 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • IHC-P

AbReview48053****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Paraffin-embedded rat brain tissue stained for Histone H3 using ab1791 at 1/8000 dilution in immunohistochemical analysis.

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • ChIP

AbReview56600****

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

The ChIP was performed with chromatin from mouse gut cell lysate and ab1791 at 1/250 dilution.

Negative control : No antibody was used (right bar).

The immunoprecipitated DNA was quantified by real time PCR.

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • WB

Project4211****

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

ab1791 is tested in western blot on a range of species. We recommend loading higher amounts of protein (20-30ug) to increase the signal in yeast lysates

All lanes:

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 3:

Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

Lane 4:

S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

Lane 5:

S.pombe Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • WB

Lab

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1791 overnight at 4°C.

Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody was used for detection.

Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) at 1/1000 dilution

Lanes 1 and 4:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lanes 2 and 5:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

Lanes 3 and 6:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 10s

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • WB

AbReview53732****

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Blocked with 3% milk for 1 hour at 25°C.

Incubated with the primary antibody for 16 hours at 4°C in 3% milk in TBS-tween.

All lanes:

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle mitochondrial fraction at 20 µg

Lane 2:

Mouse skeletal muscle nuclear fraction at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/4000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 7min

This image is courtesy of an anonymous Abreview

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • ChIP

Unknown

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Chromatin from Xenopus laevis oocytes was prepared according to the Abcam X-ChIP protocol.

Oocytes were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 mg of chromatin, 3 mg of ab7834 (anti-H3, light blue) and 3 μg of ab1791 (anti-H3, dark blue), and 20 ml of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow).

The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
  • WB

Collaborator

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

Rabbit polyclonal to Histone H3 (ab1791) at 1/5000 on S. cerevisiae whole cell lysate (40 ug per lane).

Protein resolved on 15% SDS-PAGE gel. After transfer to PVDF membrane, blots were blocked in 1X PBS, 0.1% Tween-20, and 5% milk. ab1791 was diluted in 5 ml blocking buffer at 1/5000. Blots plus primary antibodies were either incubated overnight at 4°C or at RT for 2 hours. Blots were washed 6X for 10 minutes each in PBS with 0.1% Tween-20 before addition of secondary antibodies. Secondary antibodies were diluted 1/2,000 in blocking buffer and incubated with blots for 2 hours at RT. Secondary blots were washed 4X for 10 minutes each in PBS with 0.1% Tween-20 and 2X for 10 minutes each in PBS.

All lanes:

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791)

Predicted band size: 15 kDa

false

This image is courtesy of John E. Mueller and J. Ruth German (Mary Bryk lab)

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Indian muntjac, Schizosaccharomyces pombe

アプリケーション

IP, ChIP, WB, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. Based only on sequence homology, we expect the antibody to react with multiple variants of H3 such as H3.1, H3.2 and H3.3.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Arabidopsis thaliana": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Candida albicans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cyanidioschyzon merolae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dictyostelium discoideum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Ferret": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Indian muntjac": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Neurospora crassa": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Oncorhynchus mykiss": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rice": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Schistosoma mansoni": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Schizosaccharomyces pombe": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Silk worm": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Toxoplasma gondii": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>We recommend Goat Anti-Rabbit IgG H&amp;L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) secondary antibody.</p>" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P, IP and WB.

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) was first used in a scientific publication in 1987 and has been cited over 4739 times in peer reviewed journals. It's performance in Western blot and ChIP in human and mouse samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) has high sensitivity and specificity.

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) has 230 independent reviews from customers.

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) specifically detects Histone H3 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg and 250 µg selling sizes.

Top cited antibody for this target with >5000 citations and >150 five star abreviews. Key marker for researchers studying chromatin structure, epigenetic regulation and histone modifications. This antibody can use used as a control in cancer research, particularly in understanding histone mutations in various tumors and is widely used in studies of gene expression, DNA repair and cell cycle regulation.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.
Biological function summary

Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.

Pathways

Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.

Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1
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文献 (5687)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1444 PubMed41068425

2025

Systematic over-expression of secondary metabolism transcription factors to reveal the pharmaceutical potential of Aspergillus nidulans.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Guo,Lakhansing Pardeshi,Longguang Qin,Chris Y Cheung,Xiaofeng Liu,Lu Fan,Chi Cheng Mok,Chirag Parsania,Zhiqiang Dong,Ben C B Ko,Kaeling Tan,Koon Ho Wong

Cell death discovery 11:450 PubMed41057307

2025

Insufficient NNMT promotes autophagy and disrupts progesterone signaling in endometrial stromal cells in recurrent implantation failure by modulating the H3K9me3-ALDH1A3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Song,Shaotong Zhao,Xianping Hou,Jiayuan Chen,Qian Zhang,Shizhen Su,Junhao Yan,Tianxiang Ni

Journal of cell communication and signaling 19:e70048 PubMed41040887

2025

Targeting the PI3K/Akt/NF-κB axis: Cluster of differentiation 5-like-mediated immunometabolic regulation of macrophage polarization in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Hemoren Yi,Nan Liu,Zhengyang Wu,Lei Li,Tingting Li,Qixiang Liu,Man Duan,Taihu Wan

Nature communications 16:8800 PubMed41038843

2025

Crotonate enhances intestinal regeneration after injury via HBO1-mediated H3K14 crotonylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Xiao,Shicheng Yu,Mengxian Zhang,Nanshan Zhong,Shan Hua,Zhi Fang,Zhe Zhang,Huidong Liu,Ronghui Tan,Yuan Liu,Ye-Guang Chen

Nature communications 16:8724 PubMed41028013

2025

Munc13-1 restoration mitigates presynaptic pathology in spinal muscular atrophy.

Applications

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Species

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Mehri Moradi,Julia Weingart,Chunchu Deng,Mahoor Nasouti,Michael Briese,Sibylle Jablonka,Markus Sauer,Michael Sendtner

Nature communications 16:8716 PubMed41028718

2025

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Noberini,Giulia Robusti,Alessandro Vai,Evelyn Oliva Savoia,Maria Giovanna Jodice,Giovanni Bertalot,Betül Çat,Isabella Pallavicini,Giuseppina Bonizzi,Maria Capra,Claudia Anna Sangalli,Federico Zambelli,Nicola Fusco,Salvatore Pece,Giulio Pavesi,Saverio Minucci,Tiziana Bonaldi

Nature communications 16:8696 PubMed41027940

2025

Engineered exosomes with Kras specific siRNA in pancreatic cancer: a phase I study with immunological correlates.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie S Kalluri,Brandon G Smaglo,Krishnan K Mahadevan,Michelle L Kirtley,Kathleen M McAndrews,Mayela Mendt,Sujuan Yang,Ana S Maldonado,Hikaru Sugimoto,Maria E Salvatierra,Luisa M Solis Soto,Cara Haymaker,Rick Finch,Mihai Gagea,Adam C Fluty,Steven J Ludtke,J Jack Lee,Abhinav K Jain,Gauri Varadhachary,Rachna T Shroff,Anirban Maitra,Elizabeth Shpall,Shubham Pant,Raghu Kalluri

Respiratory research 26:275 PubMed41024125

2025

ZNF184-mediated transcriptional activation of SAE1 drives the cell cycle entry and immune evasion in non-small cell lung cancer.

Applications

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Species

Unspecified reactive species

Feng Shi,Luquan Zhang,Chunli Wang,Qingwei Meng

Nature communications 16:8609 PubMed41022764

2025

Direct circMAN1A2(2,3,4,5)-CENPB mRNA interaction regulates cell proliferation and cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Cao,Guo-Hua Yuan,Shi-Meng Cao,Yu-Xin Liu,Chu-Xiao Liu,Yi-Feng Xu,Jia Wei,Yi-Lin Guo,Ling-Ling Chen,Li Yang

eLife 14: PubMed41026782

2025

Telomeres control human telomerase () expression through non-telomeric TRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Antara Sengupta,Soujanya Vinayagamurthy,Dristhi Soni,Rajlekha Deb,Ananda Kishore Mukherjee,Subhajit Dutta,Jushta Jaiswal,Mukta Yadav,Shalu Sharma,Sulochana Bagri,Shuvra Shekhar Roy,Priya Poonia,Ankita Singh,Divya Khanna,Amit Kumar Kumar Bhatt,Akshay Sharma,Suman Saurav,Rajender K Motiani,Shantanu Chowdhury
View all publications
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代替製品

Primary Antibodies

AB176842

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade

RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (AB176842)

  • 5
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Primary Antibodies

AB213257

Anti-Histone H3 antibody [EPR21228] - Nuclear Marker

Advanced Validation|Recombinant

ChIP - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (AB213257)

  • 0
  • 0
Primary Antibodies

AB201456

Anti-Histone H3 antibody [EPR17785] - Nuclear Marker

Advanced Validation|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (AB201456)

  • 4
  • 5
Primary Antibodies

AB176916

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916)

  • 4
  • 14
Primary Antibodies

AB32356

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

  • 5
  • 14
Primary Antibodies

AB52946

Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (AB52946)

  • 5
  • 12
Primary Antibodies

AB176843

Anti-Histone H3 (citrulline R2) antibody [EPR17703]

RabMAb|Advanced Validation|Recombinant

Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843)

  • 5
  • 1
Primary Antibodies

AB219407

Anti-Histone H3 (citrulline R17) antibody [EPR20358-120]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

  • 5
  • 1
Primary Antibodies

AB277918

Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y]

RabMAb|Recombinant

Immunocytochemistry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (AB277918)

  • 0
  • 0
Primary Antibodies

AB277919

Alexa Fluor® 647 Anti-Histone H3 (acetyl K14) antibody [EP964Y]

RabMAb|Recombinant

Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (AB277919)

  • 0
  • 0
Primary Antibodies

AB192985

Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade (AB192985)

  • 4
  • 14

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB10158

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

  • 4
  • 21
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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