AB24834

Anti-Histone H3 抗体 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade

Name: Anti-Histone H3 抗体 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)
Brand: Abcam Limited
SKU: AB24834
Price: 86000 JPY
Availability: InStock
Rating: 5 (6 reviews)

Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade

(6 Reviews)

(68 Publications)

Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, IP, ChIP. Suitable for Human, Mouse, Rat.

- Over 50 publications
- Trusted since 2005

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

3 Images

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Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 2:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Testis (Rat) Tissue Lysate - normal tissue (ab29388) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

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Exposure time: 3min

ChIP

Lab

ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 5μg of ab24834 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Project

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/mL

All lanes:

HeLa histone prep at 1 µg/mL

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

mAbcam 24834

アイソタイプ

IgG3

軽鎖のタイプ

kappa

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5 µg for 25 µg chromatin", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Human, Mouse, Rat samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is often used as a loading control in Western blot. It specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) was first used in a scientific publication in 2005 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) has over 5 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1

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文献 (68)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8647 PubMed41028752

2025

Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Ozkan,Mitzy Rios de Anda,Elisa Hall-Ponsele,Maria Rosa Portero Migueles,Amani Alshaikh,Marta Hanzevacki,Moriyah Naama,Katharine Furlong,Gareth A Roberts,Meryam Beniazza,My Linh Huynh,Michael R O'Dwyer,Sonia Yiakoumi,Christos Spanos,Hazar Yassen,Keisuke Kaji,Hitoshi Niwa,Yosef Buganim,Sally Lowell,Abdenour Soufi

Antioxidants (Basel, Switzerland) 14: PubMed40867843

2025

Gender-Dependent Cognitive and Metabolic Benefits Due to Glyoxalase 1 (Glo1) Overexpression in Age-Accelerated SAMP8 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alcir Luiz Dafre,Taketo Taguchi,Yelena Dayn,Antonio Currais,Pamela Maher

Journal of fungi (Basel, Switzerland) 11: PubMed39852477

2025

Direct and Indirect Protein Interactions Link FUS Aggregation to Histone Post-Translational Modification Dysregulation and Growth Suppression in an ALS/FTD Yeast Model.

Applications

Unspecified application

Species

Unspecified reactive species

Seth A Bennett,Samantha N Cobos,Raven M A Fisher,Elizaveta Son,Rania Frederic,Rianna Segal,Huda Yousuf,Kaitlyn Chan,David K Dansu,Mariana P Torrente

Molecular & cellular proteomics : MCP 24:100908 PubMed39842777

2025

Isolation of Proteins on Chromatin Reveals Signaling Pathway-Dependent Alterations in the DNA-Bound Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyu Wang,Azmal Ali Syed,Jeroen Krijgsveld,Gianluca Sigismondo

Cell reports 44:115213 PubMed39817906

2025

Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Robert S Porter,Sojin An,Maria C Gavilan,Masayoshi Nagai,Yumie Murata-Nakamura,Bo Zhou,Katherine M Bonefas,Olivier Dionne,Jeru Manoj Manuel,Joannie St-Germain,Suzanne Gascon,Jacqueline Kim,Liam Browning,Benoit Laurent,Uhn-Soo Cho,Shigeki Iwase

Circulation research 135:910-932 PubMed39279670

2024

Chronic Activation of Tubulin Tyrosination Improves Heart Function.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Pietsch,Christina Y Chen,Svenja Kupsch,Lucas Bacmeister,Birgit Geertz,Marisol Herrera-Rivero,Bente Siebels,Hannah Voß,Elisabeth Krämer,Ingke Braren,Dirk Westermann,Hartmut Schlüter,Giulia Mearini,Saskia Schlossarek,Jolanda van der Velden,Matthew A Caporizzo,Diana Lindner,Benjamin L Prosser,Lucie Carrier

Nature genetics 56:100-111 PubMed38049663

2023

The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandipan Brahma,Steven Henikoff

Communications biology 6:640 PubMed37316562

2023

Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor.

Applications

Unspecified application

Species

Unspecified reactive species

Mudit Chaand,Chris Fiore,Brian Johnston,Anthony D'Ippolito,Diane H Moon,John P Carulli,Jeffrey R Shearstone

Nature communications 14:2861 PubMed37208334

2023

ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tin-Lok Wong,Jia-Jian Loh,Shixun Lu,Helen H N Yan,Hoi Cheong Siu,Ren Xi,Dessy Chan,Max J F Kam,Lei Zhou,Man Tong,John A Copland,Leilei Chen,Jing-Ping Yun,Suet Yi Leung,Stephanie Ma

Cell reports 42:112380 PubMed37061916

2023

Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures.

Applications

Unspecified application

Species

Unspecified reactive species

Jack-Christophe Cossec,Tatiana Traboulsi,Sébastien Sart,Yann Loe-Mie,Manuel Guthmann,Ivo A Hendriks,Ilan Theurillat,Michael L Nielsen,Maria-Elena Torres-Padilla,Charles N Baroud,Anne Dejean

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 抗体 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade

Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (68)

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Abcam product promise