AB218553

Anti-Histone H3 (acetyl K9) 抗体 [RM161]

Anti-Histone H3 (acetyl K9) antibody [RM161]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal H3 acetyl K9 antibody. Suitable for ICC, ChIP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K9.

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

6 Images

Supplier Data

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemical analysis of HepG2 cells labeling Histone H3 (acetyl K9) using ab218553 at 1 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry analysis of HeLa cells treated with sodium butyrate labeling Histone H3 (acetyl K9) using ab218553 at 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using ab218553 at 1 ug/mL as the capture antibody and biotinylated anti-H3K9ac as the detection antibody.

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP performed on HeLa cells using ab218553 at 5ug. Real-time PCR was performed using primers specific to the gene indicated.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553) at 0.25 µg/mL

Lane 1:

HeLa cells untreated

Lane 2:

HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa

false

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ab218553 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

RM161

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, ChIP, ICC, ELISA

applications

免疫原

Synthetic Peptide within Human H3C1 acetyl K9. The exact immunogen used to generate this antibody is proprietary information.

P68431

特異性

Reacts to Histone H3 (acetyl K9). No cross reactivity with other acetylated Lysines in histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.5-2 µg/mL", "ICC-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K9

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0198024 PubMed39976465

2025

Zika virus inhibits cell death by inhibiting the expression of NLRP3 and A20.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Changyang Zhu,Yang Meng,Linliang Zhang,Cong Liu,Yali Qin,Mingzhou Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K9) 抗体 [RM161]

Anti-Histone H3 (acetyl K9) antibody [RM161]

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise