Anti-Histone H2B (formyl K120) 抗体 [EPR17701] (ab177788)
![Western blot - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-247397-anti-histone-h2b-formyl-k120-antibody-epr17701-western-blot.jpg "Western blot - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17701] to Histone H2B (formyl K120)
- Suitable for: PepArr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Histone H2B (formyl K120) antibody [EPR17701]
Histone H2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17701] to Histone H2B (formyl K120) -
由来種
Rabbit -
アプリケーション
適用あり: PepArr, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: NIH/3T3 and HeLa cell lysates. IHC-P: Human colon, mouse testis and rat testis tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17701 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177788の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| 特記事項 |
|---|
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PepArr
Use at an assay dependent concentration. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
ターゲット情報
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関連性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
see all -
別名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
画像
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Western blot - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)All lanes : Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Peptide Array - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-261506-anti-histone-h2b-formyl-k120-antibody-epr17701-peptide-array.jpg)
Peptide Array - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)ab177788 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-247396-anti-histone-h2b-formyl-k120-antibody-epr17701-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2B (formyl K120) with ab177788 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-247395-anti-histone-h2b-formyl-k120-antibody-epr17701-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Histone H2B (formyl K120) with ab177788 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse testis tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-247393-anti-histone-h2b-formyl-k120-antibody-epr17701-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Histone H2B (formyl K120) with ab177788 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat testis tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)](https://www.abcam.co.jp/ps/products/177/ab177788/Images/ab177788-6-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (formyl K120) antibody [EPR17701] (ab177788)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab177788 は論文での使用が確認できていません。