Anti-Histone H2B (acetyl K15) 抗体 [EP955Y] - ChIP Grade (ab62335)
![Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)](https://www.abcam.co.jp/ps/products/62/ab62335/Images/ab62335-552310-anti-histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-peptide-array.jpg "Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - ChIP Grade
- Suitable for: PepArr, ChIP-sequencing, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade
Histone H2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - ChIP Grade -
由来種
Rabbit -
特異性
This antibody only detects Histone H2B acetylated on Lysine 15. -
アプリケーション
適用あり: PepArr, ChIP-sequencing, WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035) or human breast carcinoma tissue; ChIP-seq: Chromatin prepared from HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP955Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62335の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| PepArr |
Use a concentration of 0.5 µg/ml.
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| ChIP-sequencing |
Use 4µg for 107 cells.
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| WB | (2) |
1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
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| IHC-P | (1) |
1/100 - 1/250.
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| 特記事項 |
|---|
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PepArr
Use a concentration of 0.5 µg/ml. |
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ChIP-sequencing
Use 4µg for 107 cells. |
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WB
1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa). |
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IHC-P
1/100 - 1/250. |
追加情報
Is unsuitable for IP.
ターゲット情報
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関連性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
see all -
別名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
画像
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Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
ab62335 at 0.5 μg/mL was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). A Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution was used as the secondary antibody. Washing buffer and time was protein Array Washing Buffer, 2*10mins and 2*5mins and blocking buffer was 5% BSA in TBST.
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.Scanner details: Innoscan 710
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![ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)](https://www.abcam.co.jp/ps/products/62/ab62335/Images/ab62335-464762-anti-histone-h2b-acetyl-k15-antibody-ep955y-chip-sequencing-hela-human.jpg)
ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of ab62335 [EP955Y]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here. -
![Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)](https://www.abcam.co.jp/ps/products/62/ab62335/Images/ab62335-75640-anti-histone-h2b-acetyl-k15-antibody-ep955y-western-blot.jpg)
Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with TSA)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
The bottom image shows beta Tubulin as a positive control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)](https://www.abcam.co.jp/ps/products/62/ab62335/Images/ab62335-75639-anti-histone-h2b-acetyl-k15-antibody-ep955y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue. -
![Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)](https://www.abcam.co.jp/ps/products/62/ab62335/Images/ab62335-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab62335 は 5 報の論文で使用されています。
- Kikuchi M et al. Epigenetic mechanisms to propagate histone acetylation by p300/CBP. Nat Commun 14:4103 (2023). PubMed: 37460559
- Xia L et al. Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells. Front Immunol 12:779667 (2021). PubMed: 35046941
- Lu M et al. ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition. Cell Metab 29:886-900.e5 (2019). PubMed: 30661930
- Dong L et al. Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth. Sci Rep 4:5432 (2014). PubMed: 24961246
- Vlaicu SI et al. Epigenetic modifications induced by RGC-32 in colon cancer. Exp Mol Pathol 88:67-76 (2010). PubMed: 19883641