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AB327611

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free

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Rabbit Recombinant Monoclonal H2A.X phospho S139 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Zebrafish samples.

別名を表示する

zgc:56329, h2ax, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free (AB327611)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free (AB327611)

ab327611 detects Histone H2A.XS139ph (phospho Ser139) protein by immunohistochemical analysis.Sample : Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green : Histone H2A.XS139ph (phospho Ser139) stained by ab327611 diluted at 1 : 100.Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

Western blot - Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free (AB327611)
  • WB

Supplier Data

Western blot - Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free (AB327611)

Untreated (-) and treated (+) zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ab327611 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Histone H2A.XS139ph (phospho Ser139) antibody [HL4533] - BSA and Azide free (ab327611) at 1/1000 dilution

Lane 1:

Untreated 3dbf zebrafish embryos at 30 µg

Lane 2:

8000 J/m2 UVC treated 3dbf zebrafish embryos at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL4533

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Zebrafish

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation (By similarity).
See full target information h2ax pS139
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Abcam product promise

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