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AB251208

Anti-Histone H2A (crotonyl K119) 抗体 [EPR18769] - BSA and Azide free

Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free

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Rabbit Recombinant Monoclonal H2A crotonyl K119 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.

別名を表示する

H2AFM, HIST1H2AB, H2AC8, H2AFA, HIST1H2AE, H2AC4, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AK119cr

2 Images
Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (AB251208)
  • WB

Supplier Data

Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (AB251208)

This data was developed using ab195467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (<a href='/products/primary-antibodies/histone-h2a-crotonyl-k119-antibody-epr18769-ab195467'>ab195467</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 30s

Dot Blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (AB251208)
  • Dot

Supplier Data

Dot Blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (AB251208)

This data was developed using ab195467, the same antibody clone in a different buffer formulation.

Dot blot analysis of H2A K119 crotonyl peptide (Lane 1), H2A K119 ubiquitinate peptide (Lane 2), H2A.X K119 ubiquitinate peptide (Lane 3), H2A K118 formyl peptide (Lane 4), H2A.X unmodified peptide (Lane 5) and H2A unmodified peptide (Lane 6), labeled using ab195467 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H+L (HRP) (ab97051) secondary antibody at 1/1000 dilution.

Blocking/Dilution buffer : 5% BSA/TBST.

Exposure time : 3 minutes.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-Histone H2A (crotonyl K119) antibody [EPR18769]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18769

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Dot, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB245367

Anti-Interferon alpha/beta receptor 1 antibody

Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody (AB245367)

  • 0
  • 0
Primary Antibodies

AB3344

Anti-SynGAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (AB3344)

  • 0
  • 1
Primary Antibodies

AB154505

Anti-DUSP23 antibody

Western blot - Anti-DUSP23 antibody (AB154505)

  • 0
  • 0
Primary Antibodies

AB198828

Anti-CCL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL1 antibody (AB198828)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

ab251208 is the carrier-free version of ab195467.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H2AC4 crK119
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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