Anti-Histone H1.5 抗体 (ab24175)
Key features and details
- Rabbit polyclonal to Histone H1.5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1.5 antibody
Histone H1.5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1.5 -
由来種
Rabbit -
特異性
This antibody is specific for Histone H1.5. Reacts with histone H1 acid-extracted from cell lines such as breast cancer cell line T47D. Also recognizes H1 in a nuclear extract or chromatin preparation. H1.5 is present in many human cell lines. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
AETATPAPVEKSPAKKKATKKAAGAGAAK -C
, corresponding to N terminal amino acids 7-35 of Human Histone H1.5 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 8% Glycerol -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24175の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 22 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3009 Human
- Omim: 142711 Human
- SwissProt: P16401 Human
- Unigene: 131956 Human
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別名
- H1 antibody
- H1 histone family member 5 antibody
- H1.5 antibody
see all
画像
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Western blot - Anti-Histone H1.5 antibody (ab24175)All lanes : Histone H1.5 antibody (ab24175)
Lane 1 : Chromatin preparation from a breast cancer cell line
Lane 2 : recombinant human H1.0
Lane 3 : recombinant human H1.1
Lane 4 : recombinant human H1.2
Lane 5 : recombinant human H1.3
Lane 6 : recombinant human H1.4
Lane 7 : recombinant human H1.5Panel B is a silver stain of the gel in panel A.
All lanes : Histone H1.5 antibody (ab24175)
Lane 1 : Chromatin preparation from a breast cancer cell line
Lane 2 : recombinant human H1.0
Lane 3 : recombinant human H1.1
Lane 4 : recombinant human H1.2
Lane 5 : recombinant human H1.3
Lane 6 : recombinant human H1.4
Lane 7 : recombinant human H1.5Panel B is a silver stain of the gel in panel A.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab24175 は 15 報の論文で使用されています。
- Serna-Pujol N et al. Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Res 50:3892-3910 (2022). PubMed: 35380694
- Liao R & Mizzen CA Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation. Epigenetics Chromatin 10:29 (2017). PubMed: 28539972
- Izquierdo-Bouldstridge A et al. Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats. Nucleic Acids Res 45:11622-11642 (2017). PubMed: 28977426
- Holmberg Olausson K et al. NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape. Sci Rep 5:16495 (2015). PubMed: 26559910
- Mayor R et al. Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions. J Biol Chem 290:7474-91 (2015). WB ; Human . PubMed: 25645921