AB24175

Anti-Histone H1.5 抗体

Name: Anti-Histone H1.5 抗体 (ab24175) | Abcam
Brand: Abcam Limited
SKU: AB24175
Price: 76000 JPY
Availability: InStock
Rating: 1 (1 reviews)

Anti-Histone H1.5 antibody

(1 Review)

(16 Publications)

Rabbit Polyclonal H1.5 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human H1-5 aa 1-50.

別名を表示する

H1F5, HIST1H1B, H1-5, Histone H1.5, Histone H1a, Histone H1b, Histone H1s-3

1 Images

Unknown

Western blot - Anti-Histone H1.5 antibody (AB24175)

All lanes : Histone H1.5 antibody (ab24175)

Lane 1 : Chromatin preparation from a breast cancer cell line
Lane 2 : recombinant human H1.0
Lane 3 : recombinant human H1.1
Lane 4 : recombinant human H1.2
Lane 5 : recombinant human H1.3
Lane 6 : recombinant human H1.4
Lane 7 : recombinant human H1.5

Panel B is a silver stain of the gel in panel A.

All lanes:

Western blot - Anti-Histone H1.5 antibody (ab24175)

Predicted band size: 22 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human H1-5 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16401

特異性

This antibody is specific for Histone H1.5. Reacts with histone H1 acid-extracted from cell lines such as breast cancer cell line T47D. Also recognizes H1 in a nuclear extract or chromatin preparation. H1.5 is present in many human cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.02% Sodium azide Constituents: 8% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Add glycerol to a final volume of 50% for extra stability and aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (By similarity). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed : 40516528). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

See full target information H1-5

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:3892-3910 PubMed35380694

2022

Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Núria Serna-Pujol,Mónica Salinas-Pena,Francesca Mugianesi,François Le Dily,Marc A Marti-Renom,Albert Jordan

Cell 184:3163-3177.e21 PubMed33964209

2021

Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Cui,Kasturi Chakraborty,Xu Anna Tang,Guolin Zhou,Kelly Q Schoenfelt,Kristen M Becker,Alexandria Hoffman,Ya-Fang Chang,Ariane Blank,Catherine A Reardon,Hilary A Kenny,Tomas Vaisar,Ernst Lengyel,Geoffrey Greene,Lev Becker

Nucleic acids research 45:11622-11642 PubMed28977426

2017

Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Izquierdo-Bouldstridge,Alberto Bustillos,Carles Bonet-Costa,Patricia Aribau-Miralbés,Daniel García-Gomis,Marc Dabad,Anna Esteve-Codina,Laura Pascual-Reguant,Sandra Peiró,Manel Esteller,Matthew Murtha,Lluís Millán-Ariño,Albert Jordan

Epigenetics & chromatin 10:29 PubMed28539972

2017

Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Liao,Craig A Mizzen

Scientific reports 5:16495 PubMed26559910

2015

NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Holmberg Olausson,Tamador Elsir,Kaveh Moazemi Goudarzi,Monica Nistér,Mikael S Lindström

Nucleic acids research 43:10200-12 PubMed26319017

2015

Nucleosome compaction facilitates HP1γ binding to methylated H3K9.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Mishima,Chanika D Jayasinghe,Kai Lu,Junji Otani,Masahiro Shirakawa,Toru Kawakami,Hironobu Kimura,Hironobu Hojo,Peter Carlton,Shoji Tajima,Isao Suetake

The Journal of biological chemistry 290:7474-91 PubMed25645921

2015

Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.

Applications

Species

Human

Regina Mayor,Andrea Izquierdo-Bouldstridge,Lluís Millán-Ariño,Alberto Bustillos,Cristina Sampaio,Neus Luque,Albert Jordan

The Journal of biological chemistry 289:34601-19 PubMed25349213

2014

Loss of nucleolar histone chaperone NPM1 triggers rearrangement of heterochromatin and synergizes with a deficiency in DNA methyltransferase DNMT3A to drive ribosomal DNA transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Holmberg Olausson,Monica Nistér,Mikael S Lindström

Cancer research 74:6463-73 PubMed25205099

2014

Histone h1.3 suppresses h19 noncoding RNA expression and cell growth of ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Medrzycki,Yunzhe Zhang,Weijia Zhang,Kaixiang Cao,Chenyi Pan,Nathalie Lailler,John F McDonald,Eric E Bouhassira,Yuhong Fan

FEBS letters 588:2353-62 PubMed24873882

2014

Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Michel Terme,Lluís Millán-Ariño,Regina Mayor,Neus Luque,Andrea Izquierdo-Bouldstridge,Alberto Bustillos,Cristina Sampaio,Jordi Canes,Isaura Font,Núria Sima,Mónica Sancho,Laura Torrente,Sonia Forcales,Alicia Roque,Pere Suau,Albert Jordan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H1.5 抗体

Anti-Histone H1.5 antibody

Western blot - Anti-Histone H1.5 antibody (AB24175)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (16)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise