AB134914

Anti-Histone H1.0 抗体 [EPR6536]

Name: Anti-Histone H1.0 抗体 [EPR6536] (ab134914) | Abcam
Brand: Abcam Limited
SKU: AB134914
Price: 45000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Histone H1.0 antibody [EPR6536]

RabMAb
Recombinant
KO Validated
詳細を見る

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal H1.0 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

別名を表示する

H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.

Lab

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Lanes 1 - 3 : Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 A431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

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Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

BcPC3 cel lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 21 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6536

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

See full target information H1-0

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:441-459 PubMed38765203

2024

Histone H1.0 couples cellular mechanical behaviors to chromatin structure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaishuai Hu,Douglas J Chapski,Natalie D Gehred,Todd H Kimball,Tatiana Gromova,Angelina Flores,Amy C Rowat,Junjie Chen,René R Sevag Packard,Emily Olszewski,Jennifer Davis,Christoph D Rau,Timothy A McKinsey,Manuel Rosa-Garrido,Thomas M Vondriska

Journal of extracellular vesicles 13:e12430 PubMed38602325

2024

Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Seltmann,Britta Hettich,Seraina Abele,Selina Gurri,Valeria Mantella,Jean-Christophe Leroux,Sabine Werner

Nature communications 11:1792 PubMed32286289

2020

Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Morales Torres,Mary Y Wu,Sebastijan Hobor,Elanor N Wainwright,Matthew J Martin,Harshil Patel,William Grey,Eva Grönroos,Steven Howell,Joana Carvalho,Ambrosius P Snijders,Michael Bustin,Dominique Bonnet,Paul D Smith,Charles Swanton,Michael Howell,Paola Scaffidi

EMBO molecular medicine 12:e11466 PubMed32150356

2020

A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Cangkrama,Mateusz Wietecha,Nicolas Mathis,Rin Okumura,Luca Ferrarese,Dunja Al-Nuaimi,Maria Antsiferova,Reinhard Dummer,Metello Innocenti,Sabine Werner

Frontiers in immunology 10:2176 PubMed31572386

2019

Aggregated NETs Sequester and Detoxify Extracellular Histones.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Knopf,Moritz Leppkes,Georg Schett,Martin Herrmann,Luis E Muñoz

Journal of clinical biochemistry and nutrition 64:36-44 PubMed30705510

2019

Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Fukui,Shunsuke Okihiro,Yuuka Ohfuchi,Minae Hashimoto,Yugo Kato,Naoki Yoshida,Kaho Mochizuki,Hiroki Tsumoto,Yuri Miura

The Journal of experimental medicine 215:1853-1868 PubMed29930104

2018

Trim33 mediates the proinflammatory function of Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Tanaka,Yu Jiang,Gustavo J Martinez,Kentaro Tanaka,Xiaowei Yan,Tomohiro Kurosaki,Vesa Kaartinen,Xin-Hua Feng,Qiang Tian,Xiaohu Wang,Chen Dong

PloS one 9:e104323 PubMed25116007

2014

Activation of nuclear factor kappa B in the hepatic stellate cells of mice with schistosomiasis japonica.

Applications

Species

Mouse

Xing He,Guangbin Pu,Rui Tang,Dongmei Zhang,Weiqing Pan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H1.0 抗体 [EPR6536]

Anti-Histone H1.0 antibody [EPR6536]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

製品プロトコール

ターゲットの情報

文献 (8)

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Abcam product promise