Anti-Histone H1.0 抗体 (ab154111)
Key features and details
- Rabbit polyclonal to Histone H1.0
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Histone H1.0 antibody
Histone H1.0 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1.0 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human Histone H1.0 aa 1-194. The exact sequence is proprietary.
Database link: P07305 -
ポジティブ･コントロール
- HepG2 whole cell lysate; HepG2 nuclear lysate; U20S cells; SkHep1 xenograft; C2C12 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
|
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| IHC-P |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
翻訳後修飾
Phosphorylated on Ser-17 in RNA edited version. -
細胞内局在
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Omim: 142708 Human
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- Unigene: 745024 Human
- Unigene: 24350 Mouse
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別名
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
画像
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Western blot - Anti-Histone H1.0 antibody (ab154111)All lanes : Anti-Histone H1.0 antibody (ab154111) at 1/5000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody (ab154111)Immunofluorescent analysis of paraformaldehyde fixed U20S cells labeling Histone H1 with ab154111 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2000. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (ab154111)Immunohistochemical analysis of paraffin embedded SkHep1 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (ab154111)Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154111 は論文での使用が確認できていません。