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遺伝子ノックアウト細胞株 検証済

Anti-Histone H1.0 抗体 [34] (ab11079)

Reviews (2) Submit a question References (34)
Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [34] (ab11079)
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)

Key features and details

  • Mouse monoclonal [34] to Histone H1.0
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Histone H1.0 antibody [34]
    Histone H1.0 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [34] to Histone H1.0

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mouse, Rat, Xenopus laevis, Vertebrata非交差種: Bird

  • 免疫原

    Full length native protein (purified) corresponding to Cow Histone H1.0.

  • エピトープ

    This antibody recognises an epitope within aa20-30. Proline 26 is not essential for recognition by the antibody. See Gorka et al. 1998 for more information.

  • ポジティブ･コントロール

    • WB: A431, MCF7 and HeLa cell lysate; Histone H1.0 (Human) - Recombinant Protein. IHC-P: Human breast adenocarcinama tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    34

  • ミエローマ

    NS1/1-Ag4-1

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11079の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

  • 配列類似性

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.

  • 翻訳後修飾

    Phosphorylated on Ser-17 in RNA edited version.

  • 細胞内局在

    Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

画像

  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    All lanes : Anti-Histone H1.0 antibody [34] (ab11079) at 1/500 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : H1F0 knockout A431 cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab11079 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab11079 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11079 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [34] (ab11079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [34] (ab11079)

    IHC image of Histone H1.0 staining in a formalin fixed, paraffin embedded human breast adenocarcinama tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11079, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    All lanes : Anti-Histone H1.0 antibody [34] (ab11079) at 1 µg/ml

    Lane 1 : Histone H1.0 (Human) - Recombinant Protein
    Lane 2 : Histone H1.2 (Human) - Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Anti-Histone H1.0 antibody [34] (ab11079) at 1 µg/ml + HeLa (Triton-enriched) Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?
    Additional bands at: 120 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Western blot - Anti-Histone H1.0 antibody [34] (ab11079)

    Western blot using ab11079 on HeLa whole cell extract (30 µg/lane).

    Exposure 5 sec using ECL.
    12.5% SDS-PAGE gel.

  • Western blot - Anti-Histone H1.0 antibody [34] (ab11079)
    Western blot - Anti-Histone H1.0 antibody [34] (ab11079)Dr Albert Jordan, Center for Genomic Regulation (CRG), Spain.

    Mouse monoclonal to Histone H1.0 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure.

    Dilution 1/1000.

    Recombinant H1 isoforms courtesy of Dr Nicole Happel.

    (see attached review)

参考文献 (34)

ab11079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11079 は 34 報の論文で使用されています。

  • Gómora-García JC  et al. Effect of the Ketone Body, D-β-Hydroxybutyrate, on Sirtuin2-Mediated Regulation of Mitochondrial Quality Control and the Autophagy-Lysosomal Pathway. Cells 12:N/A (2023). PubMed: 36766827
  • Bhandari SK  et al. Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1. Sci Rep 13:4363 (2023). PubMed: 36928068
  • Ozgencil M  et al. The linker histone H1-BRCA1 axis is a crucial mediator of replication fork stability. Life Sci Alliance 6:N/A (2023). PubMed: 37364916
  • Serna-Pujol N  et al. Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Res 50:3892-3910 (2022). PubMed: 35380694
  • Yang Z  et al. Forsythiaside Protected H9c2 Cardiomyocytes from H2O2-Induced Oxidative Stress and Apoptosis via Activating Nrf2/HO-1 Signaling Pathway. Int Heart J 63:904-914 (2022). PubMed: 36184550
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Histone H1.0 antibody [34]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
2 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 17 2012

Western blot abreview for Anti-Histone H1.0 antibody [34]

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC 5VA - Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC 5VA - Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 10 2009

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