Anti-Histidine decarboxylase 抗体 [EPR26392-79]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RBL-2H3 (rat Peripheral blood basophil) cells labelling Histidine decarboxylase with ab315444 at 1/50 (10.18 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in RBL-2H3 cell line.
Negative control : S16 .
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labelling with ab315444 at 1/500 (1.017 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

No staining on mouse skeletal muscle. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling with ab315444 at 1/500 (1.017 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

No staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Immunohistochemical analysis of paraffin-embedded (A) Hypothalamus tissue from wild-type C57BL/6JGpt mice and (B) Hypothalamus tissue from Hdc knockout mice labelling with ab315444 at 1/500 (1.017 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Hypothalamus tissue from wild-type C57BL/6JGpt mice and no staining on (B) Hypothalamus tissue from Hdc knockout mice. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Hdc-KO homozygous mice (Strain ID : T028366).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 80 µg

Lane 2:

Mouse hypothalamus tissue lysate at 80 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : huh7.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9525922).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

KU812 (human Peripheral blood basophil) whole cell lysate at 80 µg

Lane 2:

SR (human pleural effusion lymphoblast) whole cell lysate at 80 µg

Lane 3:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 53 kDa,74 kDa,15 kDa

false

Exposure time: 81s

• WB

Lab

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Hdc-KO homozygous mice (Strain ID : T028366). This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

Wild-type mouse hypothalamus tissue lysate (case 1) at 40 µg

Lane 2:

Wild-type mouse hypothalamus tissue lysate (case 2) at 40 µg

Lane 3:

Hdc knockout mouse hypothalamus tissue lysate (case 1) at 40 µg

Lane 4:

Hdc knockout mouse hypothalamus tissue lysate (case 2) at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

RBL-2H3 (rat Peripheral blood basophil) whole cell lysate at 80 µg

Lane 2:

S16 (rat Sciatic nerve epithelial-like) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa,15 kDa

false

Exposure time: 6s