Anti-HIRA/HIR 抗体 [EPR25299-11] - BSA and Azide free (ab302929)

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Western blot - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25299-11] to HIRA/HIR - BSA and Azide free
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Unconjugated

製品名

Anti-HIRA/HIR antibody [EPR25299-11] - BSA and Azide free
HIRA/HIR 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25299-11] to HIRA/HIR - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: IP, IHC-P, WBmore details
適用なし: ChIP,Flow Cyt (Intra) or ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: F9 transfected with unscrambled and scrambled (control) siRNA specifically targeting HIRA, whole cell lysates; human testis tissue lysates; mouse and rat brain and cerebellum tissue lysates; mouse lung tissue lysate. IHC-P: Mouse and rat cerebrum, mouse lung and human skin tissue. IP: F9 and HeLa whole cell lysate.
特記事項
AB302929 is the carrier-free version of AB302928.

AB302928 does not react with human or mouse samples in ICC/IF or Flow Cytometry (Intra). It does not react with mouse samples in ChIP.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.20
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25299-11
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab302929の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IP		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 112 kDa).

特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 112 kDa).

追加情報

Is unsuitable for ChIP,Flow Cyt (Intra) or ICC/IF.

機能

Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
組織特異性

Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
配列類似性

Belongs to the WD repeat HIR1 family.
Contains 8 WD repeats.
発生段階

Expressed during embryogenesis.
翻訳後修飾

Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
細胞内局在

Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
Target information above from: UniProt accession P54198 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7290 Human
- Entrez Gene: 15260 Mouse
- Entrez Gene: 363849 Rat
- Omim: 600237 Human
- SwissProt: P54198 Human
- SwissProt: Q61666 Mouse
- Unigene: 474206 Human
- Unigene: 15694 Mouse
- Unigene: 453041 Mouse
see all
別名
- DGCR1 antibody
- DiGeorge critical region gene 1 antibody
- HIR antibody
- HIR histone cell cycle regulation defective homolog A antibody
- HIRA antibody
- HIRA protein antibody
- HIRA_HUMAN antibody
- Histone cell cycle regulator antibody
- Protein HIRA antibody
- TUP 1 antibody
- TUP1 like enhancer of split protein 1 antibody
- TUP1-like enhancer of split protein 1 antibody
- TUPLE1 antibody
see all

Western blot - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution

Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate
Lane 2 : F9 transfected with siRNA specifically targeting HIRA, whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer: 5% NFDM/TBST.

The bands beneath the target band (110KD) are likely to be degradation.

Exposure time: 7.75 seconds.
Western blot - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human lung tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat brain tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer: 5% NFDM/TBST.

Exposure times: Lane 1-3: 114 seconds; Lane 4 and 5: 37 seconds.

Low expression: human liver, human lung (PMID: 9063745).
The bands beneath the target band (110KD) are likely to be degradation.
Western blot - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Rat cerebellum tissue lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 48 seconds

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer: 5% NFDM/TBST.

The bands beneath the target band (110KD) are likely to be degradation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on mouse lung. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on human skin. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

HIRA/HIR was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate 10 µg with ab302928 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab302928 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: F9 whole cell lysate 10 µg

Lane 2: ab302928 IP in F9 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302928 in F9 whole cell lysate

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.

The bands beneath the target band (110KD) are likely to be degradation.
Immunoprecipitation - Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

This data was developed using AB302928, the same antibody clone in a different buffer formulation.

HIRA/HIR was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab302928 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab302928 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10 µg

Lane 2: ab302928 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302928 in HeLa whole cell lysate

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.
Anti-HIRA/HIR antibody [EPR25299-11] (BSA and Azide free) (AB302929)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab302929 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302929 は論文での使用が確認できていません。

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

発生段階

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A