Anti-Hippocalcin 抗体 (ab168214)
Key features and details
- Mouse polyclonal to Hippocalcin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hippocalcin antibody
Hippocalcin 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to Hippocalcin -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Chicken, Cow, Pig, Xenopus laevis, Orangutan, Zebra finch
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免疫原
Recombinant full length protein, corresponding to amino acids 1-193 of Human Hippocalcin (NP_002134, UniProt ID: P84074)
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ポジティブ･コントロール
- Recombinant Human Hippocalcin protein (ab132300) can be used as a positive control in WB. Lysate from 293T cells transfected with Hippocalcin; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions. -
組織特異性
Brain specific. -
配列類似性
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
翻訳後修飾
Myristoylation facilitates interaction with membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 509772 Cow
- Entrez Gene: 3208 Human
- Entrez Gene: 15444 Mouse
- Entrez Gene: 100144505 Pig
- Entrez Gene: 29177 Rat
- Omim: 142622 Human
- SwissProt: Q4PL64 Cow
- SwissProt: P84074 Human
see all -
別名
- BDR 2 antibody
- BDR2 antibody
- Calcium binding protein BDR 2 antibody
see all
画像
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Western blot - Anti-Hippocalcin antibody (ab168214)All lanes : Anti-Hippocalcin antibody (ab168214) at 1 µg/ml
Lane 1 : Lysate from 293T cells transfected with Hippocalcin
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Hippocalcin antibody (ab168214)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Hippocalcin using ab168214 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab168214 は論文での使用が確認できていません。
