Anti-HIPPI/IFT57 抗体 (ab154601)
Key features and details
- Rabbit polyclonal to HIPPI/IFT57
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HIPPI/IFT57 antibody -
製品の詳細
Rabbit polyclonal to HIPPI/IFT57 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human HIPPI/IFT57 aa 227-429.
Database link: Q9NWB7 -
ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2 and Raji whole cell lysates. HeLa cells.
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特記事項
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154601の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. -
組織特異性
Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level). -
配列類似性
Belongs to the IFT57 family. -
ドメイン
The pseudo DED region (pDED) meadiates the interaction with HIP1. -
細胞内局在
Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia. - Information by UniProt
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参照データベース
- Entrez Gene: 531436 Cow
- Entrez Gene: 55081 Human
- Entrez Gene: 73916 Mouse
- Entrez Gene: 303968 Rat
- Omim: 606621 Human
- SwissProt: Q5EA95 Cow
- SwissProt: Q9NWB7 Human
- SwissProt: Q8BXG3 Mouse
see all -
別名
- CDNA FLJ10147 fis clone HEMBA1003369 antibody
- Dermal papilla derived protein 8 antibody
- Dermal papilla-derived protein 8 antibody
see all
画像
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Western blot - Anti-HIPPI/IFT57 antibody (ab154601)Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (ab154601)Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab154601)Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154601 は論文での使用が確認できていません。