Anti-HIF3 alpha/IPAS 抗体 (ab2165)
Key features and details
- Rabbit polyclonal to HIF3 alpha/IPAS
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-HIF3 alpha/IPAS antibody
HIF3 alpha/IPAS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HIF3 alpha/IPAS -
由来種
Rabbit -
特異性
A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Mouse HIF3 alpha/IPAS aa 550-650 conjugated to keyhole limpet haemocyanin.
Database link: Q0VBL6 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity-purified antibody is directed against mouse Hif3alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
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| WB |
1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| IHC-P |
Use at an assay dependent concentration. PubMed: 23451260
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| 特記事項 |
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WB
1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
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IHC-P
Use at an assay dependent concentration. PubMed: 23451260 |
ターゲット情報
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機能
Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. -
組織特異性
Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 53417 Mouse
- Entrez Gene: 64345 Rat
- SwissProt: Q0VBL6 Mouse
- SwissProt: Q9JHS2 Rat
- Unigene: 135110 Mouse
- Unigene: 161814 Rat
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別名
- Basic-helix-loop-helix-PAS protein MOP7 antibody
- bHLHe17 antibody
- Class E basic helix-loop-helix protein 17 antibody
see all
画像
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Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml
Secondary
Goat-anti-Rabbit at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab2165 は 4 報の論文で使用されています。
- Mao Y et al. Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation. Cell Res 34:13-30 (2024). PubMed: 38163844
- Wang Y et al. Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3a. Biosci Rep 39:N/A (2019). PubMed: 31652442
- Huang Y et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
- Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655