Anti-HIF-1 alpha 抗体 [EP1215Y] (ab51608)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1215Y] to HIF-1 alpha
- Suitable for: ICC/IF, ChIC/CUT&RUN-seq, Flow Cyt (Intra), IP, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HIF-1 alpha antibody [EP1215Y]
HIF-1 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1215Y] to HIF-1 alpha -
由来種
Rabbit -
特異性
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アプリケーション
適用あり: ICC/IF, ChIC/CUT&RUN-seq, Flow Cyt (Intra), IP, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: DFO treated HeLa nuclear lysate (ab180880), Ramos cell lysate treated with Cocl2. IHC-P: Human ovarian and breast carcinoma, colonic adenocarcinoma and squamous cell cervical carcinoma tissues. Human gastric cancer and CRC tumour tissue ICC/IF: DFO treated Hela cells, Cocl2 treated HeLa cells and baicalein treated HepG2 cells Flow Cyt (intra): DFO treated HeLa cells IP: DFO treated HeLa nuclear lysate ChIC/CUT&RUN-Seq: HeLa cells.
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特記事項
For Mouse specific Hif-1-alpha rabbit monoclonal antibody, please see ab179483 (clone ID: EPR16897).
ab179483 has been confirmed for Mouse sample in WB.
We have mixed customer feedback towards the rat specificity so we are unable to confirm and guarantee its performance with rat samples. Please contact technical team for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 2.24 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1215Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51608の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5 µg |
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Flow Cyt (Intra) |
1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
Use a concentration of 5 µg/ml.
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WB | (10) |
1/100 - 1/1000. Predicted molecular weight: 93 kDa.
The antibody only works in hypoxic cell and tissue lysates. For Mouse specific Hif-1-alpha rabbit monoclonal antibody, please see ab179483 (clone ID: EPR16897). ab179483 has been confirmed for mouse samples in WB. Compared with ab51608, ab308433 has higher sensitivity, we recommend ab308433 as an alternative for testing HIF-1 alpha in western blot. |
IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For IHC antigen retrieval – See protocols IHC Antigen Retrieval Protocol. |
特記事項 |
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ICC/IF
1/500. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5 µg |
Flow Cyt (Intra)
1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
Use a concentration of 5 µg/ml. |
WB
1/100 - 1/1000. Predicted molecular weight: 93 kDa. The antibody only works in hypoxic cell and tissue lysates. For Mouse specific Hif-1-alpha rabbit monoclonal antibody, please see ab179483 (clone ID: EPR16897). ab179483 has been confirmed for mouse samples in WB. Compared with ab51608, ab308433 has higher sensitivity, we recommend ab308433 as an alternative for testing HIF-1 alpha in western blot. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For IHC antigen retrieval – See protocols IHC Antigen Retrieval Protocol. |
ターゲット情報
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機能
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. -
組織特異性
Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
ドメイン
Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID). -
翻訳後修飾
In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. - Information by UniProt
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参照データベース
- Entrez Gene: 3091 Human
- Omim: 603348 Human
- SwissProt: Q16665 Human
- Unigene: 597216 Human
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別名
- ARNT interacting protein antibody
- ARNT-interacting protein antibody
- Basic helix loop helix PAS protein MOP1 antibody
see all
画像
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 DMOG (0 mM, 4 h) nuclear cell lysate
Lane 2 : Wild-type HCT 116 treated DMOG (1 mM, 4 h) nuclear cell lysate
Lane 3 : HIF1A knockout HCT 116 DMOG (0 mM, 4 h) nuclear cell lysate
Lane 4 : HIF1A knockout HCT 116 treated DMOG (1 mM, 4 h) nuclear cell lysate
Lane 5 : Wild-type HCT 116 DMOG (0 mM, 4 h) cell lysate
Lane 6 : Wild-type HCT 116 treated DMOG (1 mM, 4 h) cell lysate
Lane 7 : HIF1A knockout HCT 116 DMOG (0 mM, 4 h) cell lysate
Lane 8 : HIF1A knockout HCT 116 treated DMOG (1 mM, 4 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Western blot: Anti-HIF1A antibody [EP1215Y] (ab51608) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab51608 was shown to bind specifically to HIF1A. A band was observed at 100 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in HIF1A knockout cell line. To generate this image, wild-type and HIF1A knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/1000 dilution
Lane 1 : Wild-type HCT116 DMOG (0 mM, 4 h) cell lysate
Lane 2 : Wild-type HCT116 treated DMOG (1 mM, 4 h) cell lysate
Lane 3 : HIF1A knockout HCT116 DMOG (0 mM, 4 h) cell lysate
Lane 4 : HIF1A knockout HCT116 treated DMOG (1 mM, 4 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Western blot: Anti-HIF1A antibody [EP1215Y] (ab51608) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab51608 was shown to bind specifically to HIF1A. A band was observed at 105 kDa in wild-type Wild-type HCT 116 DMOG (0 mM, 4 h) cell lysates with no signal observed at this size in HIF1A knockout cell line. To generate this image, wild-type and HIF1A knockout Wild-type HCT 116 DMOG (0 mM, 4 h) cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/1000 dilution (ab308433 1/1000
ab181602 1/1000000)
Lanes 1 & 3 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate
Lane 4 : HeLa treated with 0.5mM DFO for 24h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Exposure time: 10 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Compared with ab51608, ab308433 has higher sensitivity, we recommend ab308433 as an alternative for testing HIF-1 alpha in western blot. -
Flow cytometry overlay histogram showing left, HeLa treated with 1mM Deferoxamine for 24h and right, negative untreated HeLa stained with ab51608 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab51608) (1x 106 in 100μl at 0.2μg/ml (1/11000)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells treated with Cocl2 (500 μM 20h+4h) and MG-132 (10µM 4h) and 5 µg of ab51608 [EP1215Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/2000 dilution
Lane 1 : MCF-7 (normoxia)
Lane 2 : MCF-7 treated with 0.5% oxygen for 24 hours
Lysates/proteins at 30000 cells per lane.
Secondary
All lanes : Polyclonal Swine anti-rabbit IgG HRP at 1/1000 dilution
Predicted band size: 93 kDaBlocking buffer: 5% milk for 16 hours at 4°C.
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Immunohistochemical analysis of Formalin-fixed paraffin-embedded human CRC tumour tissue using ab51608 for HIF-1 alpha staining. Endogenous peroxidase of sections was inhibited by 7.5% H2O2 at room temperature
In central tumor areas of human CRCs β-catenin was typically localized at the cell membrane (A) whereas only a weak staining was observed for cytoplasmic GRP78 (B) and HIF-1 alpha staining was found to be negative (C). At the invasion front strong nuclear β-catenin was detectable indicating EMT (D, G). In corresponding regions strong cytoplasmic GRP78 expression was found (E, H). In some of the cases an intense nuclear HIF-1 alpha staining was observed (F, with hypoxia), but not in others (I, without hypoxia) (magnification 200×; scale bar: 100 µm).
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ab51608 staining HIF-1-alpha in HeLa cell line treated with Cocl2 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG(1/200) was used as the secondary antibody. Nuclei were counterstained with DAPI(right hand image).
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Overlay histogram showing HeLa untreated (Blue line) and HeLa treated (Red line - Deferoxamine, 1mM, 24 hours) cells stained with ab51608. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51608, 1/11709 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr®488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22°C.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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HIF-1-alpha was immunoprecipitated using 0.5mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with unpurified ab51608.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 110kDa; HIF1 alpha
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HeLa cells were untreated or treated with 1mM Deferoxamine (DFO) for 24h and fixed with paraformaldehyde for imaging by fluorescent microscopy. Cells were blocked and stained with 1X blocking buffer (ab126587). Unpurified ab51608 was used at 1:500. DAPI was used to label the nucleus. HIF1 alpha staining is absent in untreated cells and induced by DFO treatment. HIF1 alpha localizes to the nucleus.
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Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/100 dilution + Ramos Cells treated with Cocl2 at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 93 kDa -
ab51608 staining HIF-1-alpha in Human ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
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Unpurified ab51608 staining HIF-1-alpha in HepG2 cells treated with baicalein (ab120723), by ICC/IF. Increase in HIF-1-alpha expression correlates with increased concentration of baicalein as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120723 (baicalein) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51608 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Immunohistochemical analysis of paraffin-embedded formalin-fixed human gastric cancer tissue stained for HIF-1 alpha using ab15608 at 1/600 dilution. Tissue sections were counterstained with Mayer's hematoxylin. Citrate buffer (pH 6.0) antigen retrieval using standard methodology
C. HIF-1 alpha was located mainly in the nucleus of tumor cells (positive expression ×400).
D. HIF-1 alpha original magnification ×100. -
ab51608 staining of HIF-1-alpha in untreated HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG(1/200) was used as the secondary antibody. Nuclei were counterstained with DAPI(right hand Image).
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Immunohistochemical analysis using unpurified ab51608 showing positive staining in Breast carcinoma tissue.
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Immunohistochemical analysis using unpurified ab51608 showing positive staining in Colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed via the microwave method before commencing with IHC staining protocol.
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Immunohistochemical analysis using unpurified ab51608 showing positive staining in Squamous cell cervical carcinoma tissue. Heat mediated antigen retrieval was performed via the microwave method before commencing with IHC staining protocol.
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/2000 dilution (Unpurified)
Lane 1 :HeLa nuclear extract lysate (ab150036)
Lane 2 :Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesAbcam recommends using 5% milk as the blocking agent, decreasing to 2% milk during primary and secondary incubation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1-3 and 7-9) or HIF-1-alpha siRNA (lanes 4-6 and 10-12). Cells were incubated at with 21% O2 (lanes 1-6) or 1% O2 (lanes 7-12) for 4h before lysis. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with unpurified ab51608 (1/1,000 dilution 5% milk) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
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All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/2000 dilution (unpurified)
Lane 1 : HeLa Whole Cell Lysate (untreated, negative control)
Lane 2 : HeLa DFO treated (0.5mM, 24h) Whole Cell Lysate
Lane 3 : HeLa Nuclear Cell Lysate (untreated, negative control)
Lane 4 : HeLa Nuclear DFO treated (0.5mM, 24h) Cell Lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesAbcam recommends using 5% milk as the blocking agent, decreasing to 2% milk during primary and secondary incubation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (340)
ab51608 は 340 報の論文で使用されています。
- Han Y et al. Study on the expression and function of chordin-like 1 in oral squamous cell carcinoma. Oral Dis 29:2034-2051 (2023). PubMed: 35510812
- Li YK et al. Validation of ESM1 Related to Ovarian Cancer and the Biological Function and Prognostic Significance. Int J Biol Sci 19:258-280 (2023). PubMed: 36594088
- Zheng S et al. HIF‑1α inhibits ferroptosis and promotes malignant progression in non‑small cell lung cancer by activating the Hippo‑YAP signalling pathway. Oncol Lett 25:90 (2023). PubMed: 36817050
- Zhang L et al. Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor Microenvironments. Cancers (Basel) 15:N/A (2023). PubMed: 36980689
- Terzic J et al. Hypoxia-inducible factor 1A inhibition overcomes castration resistance of prostate tumors. EMBO Mol Med 15:e17209 (2023). PubMed: 37070472