AB228649

Anti-HIF-1 alpha 抗体 - C-terminal

Name: Anti-HIF-1 alpha 抗体 - C-terminal (ab228649) | Abcam
Brand: Abcam Limited
SKU: AB228649
Price: 59000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-HIF-1 alpha antibody - C-terminal

(2 Reviews)

(36 Publications)

Anti-HIF-1 alpha antibody - C-terminal (ab228649) is a rabbit polyclonal antibody detecting HIF-1 alpha in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Human, Mouse.

- Over 10 publications

別名を表示する

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

9 Images

Supplier Data

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Flag-tagged HIF-1 alpha, whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 92 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF-1 alpha (green) using ab228649 at 1/500 dilution in ICC/IF.

Counterstain : Phalloidin, a cytoskeleton marker, at 1/100 dilution (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Paraffin-embedded human kidney cancer tissue stained for HIF-1 alpha using ab228649 at 1/500 dilution in immunohistochemical analysis.

ChIP - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

ChIP

Supplier Data

ChIP - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either normal rabbit IgG or ab228649. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Supplier Data

Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

HIF-1 alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 μM CoCl2 for 24 hours, whole cell extract using 5 μg of ab228649. Western blot was performed from the immunoprecipitate using ab228649.

Lane 1 : Control IgG IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.
Lane 2 : ab228649 IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.

All lanes:

Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

Predicted band size: 92 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

4% paraformaldehyde-fixed, treated and mock-treated NIH/3T3 (mouse embryo fibroblast cell line) cells stained for HIF-1 alpha (green) using ab228649 at 1/200 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution

Lane 1:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) treated with 1% O2 for 24 hours, whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 500 µM CoCl2 for 24 hours, whole cell extract at 30 µg

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/500 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) treated with 500 µM CoCl2 for 24 hours, whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 92 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ChIP, WB, IHC-P, ICC/IF, IP

applications

免疫原

Recombinant Fragment Protein within Human HIF1A. The exact immunogen used to generate this antibody is proprietary information.

Q16665

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/570", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate.Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended <a href='/products/tissue-lysates/hela-dfo-treated-05mm-24h-nuclear-lysate-ab180880'>ab180880</a>. The cell fractionation kit can also be purchased separately <a href='/products/sample-preparation-kits/cell-fractionation-kit-standard-ab109719'>ab109719</a>. Negative Control: Most normal tissues or cells, other than kidney or heart.The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate.Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended <a href='/products/tissue-lysates/hela-dfo-treated-05mm-24h-nuclear-lysate-ab180880'>ab180880</a>. The cell fractionation kit can also be purchased separately <a href='/products/sample-preparation-kits/cell-fractionation-kit-standard-ab109719'>ab109719</a>. Negative Control: Most normal tissues or cells, other than kidney or heart.The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-HIF-1 alpha antibody - C-terminal (ab228649) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse samples.

What is the molecular weight of HIF-1 alpha?
Anti-HIF-1 alpha - C-terminal (ab228649) specifically detects a band for HIF-1 alpha (UniProt: Q16665) at a molecular weight of 93kDa.

Trusted by the scientific community
Anti-HIF-1 alpha - C-terminal (ab228649) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.

Biological function summary

HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.

Pathways

HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.

HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.

See full target information HIF1A

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

Obstetrics & gynecology science 68:408-417 PubMed40610038

2025

Correlation of VEGF, HIF-1α, and MMP2 expression in placental villi among patients with recurrent spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Ju,Xiaolin Hou,Yage Wang,Xiaofeng Zhang,Yan Feng

iScience 28:112576 PubMed40487422

2025

Mesoporous polydopamine@CeO nanoparticles for photoacoustic-guided photothermal therapy in suppressing arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Huazhen Liu,Jingjing Wang,Chenyang Zhao,Rui Zhang,Ming Wang,Yuxin Jiang,Desheng Sun,Yanglong Hou,Meng Yang

Journal of animal science and biotechnology 16:62 PubMed40307878

2025

Lactate induces oxidative stress by HIF1α stabilization and circadian clock disturbance in mammary gland of dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Feng,Lei Zhu,Cunman He,Ruidong Xiang,Jianxin Liu,Jie Cai,Diming Wang

Biological & pharmaceutical bulletin 48:137-143 PubMed39993744

2025

Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor, FG4592, Induces Endogenous Metallothionein3 Expression in Human Neuronal Cell Line, ReNcell CX Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuki Tsuru,Taisei Ito,Kazuki Komai,Fukuto Kunitomo,Yukie Nakayama,Takanori Murakami,Kazuki Ohuchi,Yasuhiro Shinkai,Tomoki Kimura,Nobuhiko Miura,Yoshito Kumagai,Isao Hozumi,Masatoshi Inden,Hisaka Kurita

Molecules (Basel, Switzerland) 30: PubMed39942733

2025

Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Winda Tasia,Amane Washio,Koki Yamate,Kenta Morita,Yutaro Mori,Prihardi Kahar,Ryohei Sasaki,Chiaki Ogino

Scientific reports 14:28096 PubMed39543383

2024

Hypoxia and collagen deposition in the kidneys infected with Acanthamoeba sp.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Kot,Marta Grabowska,Maciej Tarnowski,Patrycja Kupnicka,Patrycja Tomasiak,Danuta Kosik-Bogacka,Natalia Łanocha-Arendarczyk

STAR protocols 5:103276 PubMed39178111

2024

Protocol for producing a rat model of non-obese prediabetes using a mild hypercaloric diet approach.

Applications

Unspecified application

Species

Unspecified reactive species

Haneen S Dwaib,Ahemd F El-Yazbi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309885 PubMed38956900

2024

Identification of Hypoxia-ALCAM Macrophage- Exhausted T Cell Axis in Tumor Microenvironment Remodeling for Immunotherapy Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Xun,Huanran Zhou,Mingyi Shen,Yao Liu,Chengcao Sun,Yanhua Du,Zhou Jiang,Liuqing Yang,Qing Zhang,Chunru Lin,Qingsong Hu,Youqiong Ye,Leng Han

Bioactive materials 39:206-223 PubMed38827172

2024

Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Guo,Wei Zhao,Xinyu Xiao,Shanshan Liu,Liang Liu,La Zhang,Lu Li,Zhenghang Li,Zhi Li,Mengxia Xu,Qiling Peng,Jianwei Wang,Yuxian Wei,Ning Jiang

Journal of gastrointestinal oncology 15:237-249 PubMed38482232

2024

Activation of RhoA/ROCK2 signaling by hypoxia-inducible factor 1α in promoting tumor growth and metastasis in human colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kunli Du,Pengfei Wang,Jinqiang Liu,Jiahui Ren,Gaozan Zheng,Shisen Li,Ling Chen,Wei Hou,Hiroki Hashida,Fan Feng,Jianyong Zheng

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-HIF-1 alpha 抗体 - C-terminal

Anti-HIF-1 alpha antibody - C-terminal

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

ChIP - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Western blot - Anti-HIF-1 alpha antibody - C-terminal (AB228649)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (36)

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Secondary Antibodies

Primary Antibodies

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Primary Antibodies

Abcam product promise