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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-HIBADH 抗体 [EPR12525(B)] (ab172955)

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Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
  • Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12525(B)] to HIBADH
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HIBADH antibody [EPR12525(B)]
    HIBADH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12525(B)] to HIBADH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Fetal kidney, Fetal liver, 293T, HeLa, MCF-7 and HepG2 lysates. IHC-P: Human breast carcinoma and liver tissues ICC/IF: HepG2 cells. Flow Cyt (intra): 293T cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172955の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
IP
1/100 - 1/500.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
IP
1/100 - 1/500.

ターゲット情報

画像

  • Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HIBADH knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab172955 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab172955 Anti-HIBADH antibody [EPR12525(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265888 (knockout cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab172955 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunofluorescent analysis of HepG2 cells labeling HIBADH with ab172955 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HIBADH with ab172955 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Intracellular flow cytometric analysis of permeabilized 293T cellslabeling HIBADH with ab172955 at 1/10 dilution (red) compared toa rabbit IgG negative control (green). 

     

     

  • Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/10000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : 293T lysate
    Lane 4 : MCF-7 lysate
    Lane 5 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab172955 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HIBADH with ab172955 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Anti-HIBADH antibody [EPR12525(B)] (ab172955)

参考文献 (0)

ab172955 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab172955 は論文での使用が確認できていません。

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