Anti-hHR23A 抗体 (ab3836)
Key features and details
- Goat polyclonal to hHR23A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-hHR23A antibody
hHR23A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to hHR23A -
由来種
Goat -
特異性
This antibody has been shown to detect an approxiametely 60kd band in HeLa and Jurkat lysates. This is comparable in size to the band detected in HeLa in van der Spek et al. 1996. We believe this is good evidence that this antibody is detecting hHR23A.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide:
KSPSEESAPTTSPEC
, corresponding to amino acids 121-133 of Human hHR23A. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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機能
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome. -
配列類似性
Belongs to the RAD23 family.
Contains 2 UBA domains.
Contains 1 ubiquitin-like domain. -
ドメイン
The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5886 Human
- Entrez Gene: 19358 Mouse
- Omim: 600061 Human
- SwissProt: P54725 Human
- SwissProt: P54726 Mouse
- Unigene: 643267 Human
- Unigene: 485055 Mouse
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別名
- hHR 23A antibody
- hHR23A antibody
- HR23A antibody
see all
画像
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Western blot - Anti-hHR23A antibody (ab3836)
Western blot using ab3836 at 1/500.
Secondary ab: Rabbit anti Goat ab6741 1/5000
Exposure time:30 secondsLane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab3836 は論文での使用が確認できていません。