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AB323267

Anti-HEY2 抗体 [EPR29024-13]

Anti-HEY2 antibody [EPR29024-13]

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Rabbit Recombinant Monoclonal HEY2 antibody. Suitable for WB and reacts with Human, Mouse samples.

別名を表示する

BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, HEY2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, Hairy-related transcription factor 2, Protein gridlock homolog, hCHF1, bHLHb32, HESR-2, HRT-2, hHRT2

3 Images
Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 2:

HEK-293 transfected with siRNA specifically targeting HEY2 whole cell lysate at 80 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 70s

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 48 seconds, lanes 3-4 : 180 seconds

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

Untreated HEK-293 whole cell lysate at 80 µg

Lane 2:

HEK-293 treated with 10 µM MG-132 for 6 hours whole cell lysate at 80 µg

Lane 3:

Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Lane 4:

Neuro-2a treated with 10 µM MG-132 for 16 hours whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HEY1 and HEYL.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - Total Protein Control ChIP Grade (ab213204) at 1/5000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

His-tagged human HEY2 recombinant protein at 10 ng

Lane 2:

His-tagged human HEY1 recombinant protein at 10 ng

Lane 3:

His-tagged human HEYL recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,90 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29024-13

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
See full target information HEY2
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Abcam product promise

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