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リコンビナントRabMAb

Anti-HEXA 抗体 [EPR26394-74] (BSA and Azide free) (ab300448)

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Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)
  • Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)
  • Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Immunoprecipitation - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
  • Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26394-74] to HEXA - BSA and Azide free
  • Suitable for: IHC-Fr, IP, Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-HEXA antibody [EPR26394-74] (BSA and Azide free)
    HEXA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26394-74] to HEXA - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IP, Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: NIH/3T3, C2C12, PC-12 , C6 and RAW264.7 whole cell lysate. Mouse and rat testis, heart and liver tissue lysate. IHC-P: Mouse colon and rat testis tissue. ICC/IF: PC-12 and RAW 264.7 cell lines. Flow Cyt (Intra): PC-12 and RAW264.7 cell lines. IP: RAW264.7 cell line.

  • 特記事項

    Ab300448 is a carrier free version of ab300447. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300448の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity.

  • 関連疾患

    Defects in HEXA are the cause of GM2-gangliosidosis type 1 (GM2G1) [MIM:272800]; also known as Tay-Sachs disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G1 is characterized by GM2 gangliosides accumulation in the absence of HEXA activity, leading to neurodegeneration and, in the infantile form, death in early childhood. GM2G1 has an increased incidence among Ashkenazi Jews and French Canadians in eastern Quebec. It exists in several forms: infantile (most common and most severe), juvenile and adult (late onset).

  • 配列類似性

    Belongs to the glycosyl hydrolase 20 family.

  • 翻訳後修飾

    N-linked glycan at Asn-115 consists of Man(3)-GlcNAc(2).

  • 細胞内局在

    Lysosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Beta hexosaminidase alpha chain precursor antibody
      • Beta hexosaminidase subunit alpha antibody
      • Beta N acetylhexosaminidase antibody
      • Beta N acetylhexosaminidase subunit alpha antibody
      • Beta-hexosaminidase A antibody
      • Beta-hexosaminidase subunit alpha antibody
      • Beta-N-acetylhexosaminidase subunit alpha antibody
      • Hexa antibody
      • HEXA_HUMAN antibody
      • Hexosaminidase A (alpha polypeptide) antibody
      • Hexosaminidase A alpha polypeptide antibody
      • Hexosaminidase A antibody
      • Hexosaminidase subunit A antibody
      • MGC99608 antibody
      • N acetyl beta glucosaminidase antibody
      • N acetyl beta glucosaminidase subunit alpha antibody
      • N-acetyl-beta-glucosaminidase subunit alpha antibody
      • TSD antibody
      see all

    画像

    • Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      All lanes : Anti-HEXA antibody [EPR26394-74] (ab300447) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 mg/ml

      Predicted band size: 61 kDa
      Observed band size: 61, 54 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The doublet bands are the precursor form (61 kDa) of HEXA and the mature form (54 kDa) of HEXA which is consistent with that described in the literature (PMID: 27682588, PMID: 30341570).

    • Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Western blot - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      All lanes : Anti-HEXA antibody [EPR26394-74] (ab300447) at 1/1000 dilution

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse liver tissue lysate
      Lane 4 : Rat testis tissue lysate
      Lane 5 : Rat liver tissue lysate
      Lane 6 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61, 54 kDa why is the actual band size different from the predicted?



      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 

      Lane 1-5: 10 seconds
      Lane 6: 26 seconds

      The doublet bands are the precursor form (61 kDa) of HEXA and the mature form (54 kDa) of HEXA which is consistent with that described in the literature (PMID: 27682588, PMID: 30341570 ).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded rat testis labeling HEXA with ab300447 at 1/2000 dilution (0.289 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat testis. The section was incubated with ab300447 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded mouse colon labeling HEXA with ab300447 at 1/2000 dilution (0.289 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse colon. The section was incubated with ab300447 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)
      Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Immunofluorescent analysis of 80% methanol fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) labelling HEXA with ab300447 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing cytoplasmic staining in PC-12 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)
      Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (ab300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophages) labelling HEXA with ab300447 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.

    • Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol  permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) labeling HEXA with ab300447 at 1/500 dilution (Red) comared with Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an ulabeled cells without incubation with primary antibody and secondary antibody / Blue. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody. 

    • Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol  permeabilized PC-12 (Rat adrenal gland pheochromocytoma) labeling HEXA with ab300447 at 1/500 dilution (Red) comared with Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an ulabeled cells without incubation with primary antibody and secondary antibody / Blue. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody. 

    • Immunoprecipitation - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Immunoprecipitation - Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

      This data was developed using ab300447, the same antibody clone in a different buffer formulation. 

      HEXA was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with ab300447 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300447 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg (Inset)

      Lane 2: ab300447 IP in RAW264.7 whole cell lysate 10 μg

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300448 in NIH/3T3 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3.25 seconds. 

    • Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)
      Anti-HEXA antibody [EPR26394-74] (BSA and Azide free) (AB300448)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300448 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300448 は論文での使用が確認できていません。

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