Anti-HEXA 抗体 [EPR26394-74] (ab300447)
![Western blot - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486652-anti-hexa-epr2639474-western-blot.jpg "Western blot - Anti-HEXA antibody [EPR26394-74] (AB300447)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26394-74] to HEXA
- Suitable for: Flow Cyt (Intra), IHC-Fr, IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-HEXA antibody [EPR26394-74]
HEXA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26394-74] to HEXA -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-Fr, IP, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: NIH/3T3, C2C12, PC-12 , C6 and RAW264.7 whole cell lysate. Mouse and rat testis, heart and liver tissue lysate. IHC-P: Mouse colon and rat testis tissue. ICC/IF: PC-12 and RAW 264.7 cell lines. Flow Cyt (Intra): PC-12 and RAW264.7 cell lines. IP: RAW264.7 cell line.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26394-74 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300447の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| IHC-Fr |
1/50.
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| IP |
1/30.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Predicted molecular weight: 61 kDa.
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| ICC/IF |
1/50.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/500. |
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IHC-Fr
1/50. |
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IP
1/30. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Predicted molecular weight: 61 kDa. |
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ICC/IF
1/50. |
ターゲット情報
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機能
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. -
関連疾患
Defects in HEXA are the cause of GM2-gangliosidosis type 1 (GM2G1) [MIM:272800]; also known as Tay-Sachs disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G1 is characterized by GM2 gangliosides accumulation in the absence of HEXA activity, leading to neurodegeneration and, in the infantile form, death in early childhood. GM2G1 has an increased incidence among Ashkenazi Jews and French Canadians in eastern Quebec. It exists in several forms: infantile (most common and most severe), juvenile and adult (late onset). -
配列類似性
Belongs to the glycosyl hydrolase 20 family. -
翻訳後修飾
N-linked glycan at Asn-115 consists of Man(3)-GlcNAc(2). -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 15211 Mouse
- Entrez Gene: 300757 Rat
- SwissProt: P29416 Mouse
- SwissProt: Q641X3 Rat
- Unigene: 2284 Mouse
- Unigene: 92939 Rat
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別名
- Beta hexosaminidase alpha chain precursor antibody
- Beta hexosaminidase subunit alpha antibody
- Beta N acetylhexosaminidase antibody
see all
画像
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Western blot - Anti-HEXA antibody [EPR26394-74] (AB300447)All lanes : Anti-HEXA antibody [EPR26394-74] (ab300447) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 61, 54 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The doublet bands are the precursor form (61 kDa) of HEXA and the mature form (54 kDa) of HEXA which is consistent with that described in the literature (PMID: 27682588, PMID: 30341570).
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![Western blot - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486655-anti-hexa-epr2639474-western-blot-2.jpg)
Western blot - Anti-HEXA antibody [EPR26394-74] (AB300447)All lanes : Anti-HEXA antibody [EPR26394-74] (ab300447) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat testis tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 61, 54 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-5: 10 seconds; Lane 6: 26 seconds.
The doublet bands are the precursor form (61 kDa) of HEXA and the mature form (54 kDa) of HEXA which is consistent with that described in the literature (PMID: 27682588, PMID: 30341570).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486658-anti-HEXA-EPR2639474-ihcp-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (AB300447)Immunohistochemical analysis of paraffin-embedded rat testis labeling HEXA with ab300447 at 1/2000 dilution (0.289 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat testis. The section was incubated with ab300447 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486657-anti-HEXA-EPR2639474-ihcp-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody [EPR26394-74] (AB300447)Immunohistochemical analysis of paraffin-embedded mouse colon labeling HEXA with ab300447 at 1/2000 dilution (0.289 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse colon. The section was incubated with ab300447 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (ab300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486662-anti-HEXA-EPR2639474-ICC-pc12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (ab300447)Immunofluorescent analysis of 80% methanol fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) labelling HEXA with ab300447 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing cytoplasmic staining in PC-12 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (ab300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486663-anti-HEXA-EPR2639474-ICC-RAW2647.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HEXA antibody [EPR26394-74] (ab300447)Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophages) labelling HEXA with ab300447 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.
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![Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486666-anti-hexa-antibody-epr26394-74-intracellular-flow-cytomoetry-raw2647.jpg)
Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (AB300447)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) labeling HEXA with ab300447 at 1/500 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled cells without incubation with primary antibody and secondary antibody / Blue. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486669-anti-hexa-antibody-epr26394-74-intracellular-flow-cytomoetry-pc12.jpg)
Flow Cytometry (Intracellular) - Anti-HEXA antibody [EPR26394-74] (AB300447)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) labeling HEXA with ab300447 at 1/500 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled cells without incubation with primary antibody and secondary antibody / Blue. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody.
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![Immunoprecipitation - Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486671-anti-hexa-antibody-epr26394-74-immunoprecipitation-pc12.jpg)
Immunoprecipitation - Anti-HEXA antibody [EPR26394-74] (AB300447)HEXA was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with ab300447 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300447 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 whole cell lysate 10 μg (Inset)
Lane 2: ab300447 IP in RAW264.7 whole cell lysate 10 μg
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300447 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
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![Anti-HEXA antibody [EPR26394-74] (AB300447)](https://www.abcam.co.jp/ps/products/300/ab300447/Images/AB300447-486651-Benefits-of-Recombinant-antibodies.jpg)
Anti-HEXA antibody [EPR26394-74] (AB300447)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300447 は論文での使用が確認できていません。